Hampton蛋白结晶试剂盒Parabar 10312 (previously known as Paratone)

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Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

应用

  • Oil based cryoprotectant

特征

  • Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
  • Oil based cryoprotectant

描述

Sorry, Parabar 10312 is no longer available.  We’ve left this page up for reference only.  See HR2-861 Santovac Cryo Oil as a possible substitute.

抱歉,Parabar 10312 不再可用。 我们保留此页面仅供参考。 请参阅 HR2-861 Santovac Cryo Oil 作为可能的替代品。

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512.

Paratone® is a registered trademark of Chevron Corporation.

Parabar 10312 是一种粘性冷冻保护剂,适用于小分子和大分子晶体学。 1

可以在惰性气氛下用 Parabar 包覆对空气轻微不稳定的小分子化合物。 Parabar 保护的晶体样品可以在冷冻氮气流中进行低温冷却。 Parabar 也已成功用作生物大分子晶体的冷冻保护剂。在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用 Parabar 涂覆晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)向晶体的暴露和缓慢扩散。

HR2-643 Parabar 10312 不再可用,自 2021 年 5 月 11 日起停产。

HR2-862 Parabar 10312 以 5 ml 装在琥珀色玻璃小瓶中,螺旋盖盖中带有玻璃涂抹棒。

通过将 50:50 与石蜡油混合,可以降低 Parabar 的粘度,以改善易碎针状和刀片状晶体的处理。

Parabar 是聚异丁烯的混合物,Staudinger 分子量约为 20,000,含有约 20% 的聚合物。

20°C 时的折射率为 1.48487。

100°C 时运动粘度为 641 cSt。 100°C 时运动粘度比为 2.05。

清晰到略微朦胧的是外观。

晶体学文献中使用的同义词:Paratone® N、Paratone® 8277、润英联 V8512。

Paratone® 是雪佛龙公司的注册商标。

Parabar 10312

Parabar 10312 (previously known as Paratone)
Parabar 10312 (previously known as Paratone)

相关项目

  • Perfluoropolyether Cryo Oil
  • Santovac Cryo Oil
  • 全氟聚醚冷冻油
    Santovac 冷冻油

参考

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.

1. H. Hope,生物大分子的低温晶体学:一种普遍适用的方法。 晶体学报。 (1988) B44,22-26。

2. 与选择性调节剂 LGD2226 复合的人雄激素受体的配体结合域 (LBD) 的结构。 F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi 和 T. Jiang。 晶体学报。 (2006 年)。 F62, 1067-1071。

3. H.霍普,安努。 牧师生物物理学。 化学。 1990 年 19:107-126。

4. S. Parkin 和 H. Hope,J. Appl。 水晶。 (1998) 第 945-953 页。

Hampton蛋白结晶试剂盒Perfluoropolyether Cryo Oil/HR2-814

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Cryoprotectants > Perfluoropolyether Cryo Oil

Perfluoropolyether Cryo Oil全氟聚醚冷冻油

Perfluoropolyether Cryo Oil

CAT NO

HR2-814

NAME

Perfluoropolyether Cryo Oil

DESCRIPTION

1 mL

PRICE

$33.00

应用

  • Oil based cryoprotectant
  • 油基防冻剂

特征

  • Perfluoropolyether cryo oil
  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • 全氟聚醚冷冻油
    低粘度
    低表面张力
    可溶性和膜蛋白晶体的冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Perfluoropolyether Cryo Oil offers low viscosity, extremely low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Perfluoropolyether to the crystallization drop before mounting the crystal. After adding the Perfluoropolyether, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Perfluoropolyether will coat the mounted crystal. Cryogenically cool the mounted crystal.

在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。 用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气(氧气)向晶体的暴露和缓慢扩散。

全氟聚醚冷冻油具有低粘度、极低蒸气压、低表面张力、优异的化学惰性和优异的热稳定性。

将安装的晶体浸入油中以置换表面水。 或者在安装晶体之前在结晶液滴中加入少量的全氟聚醚。 添加全氟聚醚后,使用 Mounted Cryoloop 安装晶体。 从液滴中取出已镶嵌的晶体,全氟聚醚将覆盖已镶嵌的晶体。 低温冷却安装的晶体。

相关项目

  • CryoPro

参考

1. Structure of the ‘open form’ of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.
1. 构巢曲霉 3-脱氢喹酸合酶“开放形式”的结构,分辨率为 1.7 Å。 C.E.尼科尔斯,A.R. 霍金斯和 D.K. 斯塔默萨。 晶体学报 D 部分,第 60 卷,第 5 部分,第 971-973 页,2004 年 5 月。

2. H.希望,安努。 牧师生物物理学。 化学。 1990 年 19:107-126。

Hampton蛋白结晶试剂盒Santovac Cryo Oil/HR2-861

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Cryoprotectants > Santovac Cryo Oil

Santovac Cryo Oil

Santovac Cryo Oil
CAT NO

HR2-861

NAME

Santovac Cryo Oil

DESCRIPTION

1 mL

PRICE

$26.00

应用

  • Oil based cryoprotectant
  • 油基防冻剂

特征

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant
  • 低粘度
    低表面张力
    可溶性和膜蛋白晶体的冷冻保护剂
    不与大多数溶剂混溶
    无毒、非渗透性冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气(氧气)向晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后,使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶,Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE),具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

Santovac Cryo Oil

支持材料

HR2-861 Santovac Cryo Oil User Guide HR2-861 Santovac Cryo Oil SDS

相关项目

  • CryoPro

参考

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

1. 构巢曲霉 3-脱氢喹酸合酶“开放”形式的结构,分辨率为 1.7 Å,来自酶转换后生长的晶体。 C.E.尼科尔斯,A.R. 霍金斯和 D.K. 斯塔默萨。 晶体学报 D 部分,第 60 卷,第 5 部分,第 971-973 页,2004 年 5 月。

2. Caspase 激活剂人粒酶 B 的晶体结构,一种对 Asp-P1 残基具有高度特异性的蛋白酶。 E. Estebanez-Perpina 等人。 生物学。 化学,卷。 381,第 1203-1214 页,2000 年 12 月。

Hampton蛋白结晶试剂盒TMAO/HR2-857

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Products > Optimize Reagents > Optimize – Cryoprotectants > TMAO

TMAO

TMAO
CAT NO

HR2-857

NAME

4.0 M TMAO

DESCRIPTION

1 mL

PRICE

$26.00

支持材料

TMAO HR2-857 4.0 M TMAO SDS

应用

  • Water soluble cryoprotectant
  • 水溶性冷冻保护剂

特征

  • A very versatile cryoprotectant for macromolecular crystals
  • Protein stabilizer
  • TMAO can be a viable alternative when conventional cryoprotectants fail
  • Low viscosity and low surface tension
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 一种非常通用的大分子晶体冷冻保护剂
    蛋白质稳定剂
    当传统冷冻保护剂失效时,TMAO 可能是一种可行的替代方案
    低粘度和低表面张力
    无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Trimethylamine N-oxide dihydrate (TMAO)

Formulated from TMAO dihydrate in Type 1 lab water (deionized) and sterile filtered (0.22 micron)

Synonyms: TMANO, TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
Mr 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
Purity ≥ 98.0%
Measured pH range: 9.2 – 10.6 at 25°C
Measured Conductivity Range: 5.6 – 96.6 µS/cm at 25°C
Measured Refractive Index Range: 1.37660 – 1.37804 at 20°C

When using TMAO as a cryoprotectant, try soaking the crystals in 4.0 M TMAO for 10 seconds and then cryo cool directly in the cryo stream.

由 1 型实验室用水(去离子)和无菌过滤(0.22 微米)中的 TMAO 二水合物配制而成

同义词:TMANO、TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
纯度≥98.0%
测得的 pH 值范围:25°C 时 9.2 – 10.6
测得的电导率范围:25°C 时 5.6 – 96.6 µS/cm
测得的折射率范围:20°C 时为 1.37660 – 1.37804

当使用 TMAO 作为冷冻保护剂时,尝试将晶体浸泡在 4.0 M TMAO 中 10 秒,然后直接在冷冻流中冷冻冷却。

相关项目

  • CryoPro

参考

1. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Christoph Mueller-Dieckmann, Brice Kauffmann and Manfred S. Weiss. J. Appl. Cryst. (2011). 44, 433–436 doi:10.1107/S0021889811000045.

1. 三甲胺 N-氧化物作为大分子晶体学中的多功能冷冻保护剂。 Christoph Mueller-Dieckmann、Brice Kauffmann 和 Manfred S. Weiss。 J.应用。 水晶。 (2011)。 44, 433–436 doi:10.1107/S0021889811000045。