标签归档：HR2-861

Hampton蛋白结晶试剂盒Santovac Cryo Oil/HR2-861

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Products > Optimize Reagents > Optimize – Cryoprotectants > Santovac Cryo Oil

Santovac Cryo Oil

Santovac Cryo Oil
CAT NO

HR2-861

NAME

Santovac Cryo Oil

DESCRIPTION

1 mL

PRICE

$26.00

应用

  • Oil based cryoprotectant
  • 油基防冻剂

特征

  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • Not miscible with most solvents
  • Non toxic, non penetrating cryoprotectant
  • 低粘度
    低表面张力
    可溶性和膜蛋白晶体的冷冻保护剂
    不与大多数溶剂混溶
    无毒、非渗透性冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac® is a trademark of Santovac® Fluids.

在晶体安装在低温回路中后，在低温晶体学期间用于置换和减少晶体上的水（母液、试剂）量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气（氧气）向晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后，使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶，Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE)，具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

Santovac Cryo Oil

支持材料

HR2-861 Santovac Cryo Oil User Guide HR2-861 Santovac Cryo Oil SDS

相关项目

  • CryoPro

参考

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

1. 构巢曲霉 3-脱氢喹酸合酶“开放”形式的结构，分辨率为 1.7 Å，来自酶转换后生长的晶体。 C.E.尼科尔斯，A.R. 霍金斯和 D.K. 斯塔默萨。 晶体学报 D 部分，第 60 卷，第 5 部分，第 971-973 页，2004 年 5 月。

2. Caspase 激活剂人粒酶 B 的晶体结构，一种对 Asp-P1 残基具有高度特异性的蛋白酶。 E. Estebanez-Perpina 等人。 生物学。 化学，卷。 381，第 1203-1214 页，2000 年 12 月。