The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

CrystalCap

CrystalCap

CrystalCap Vial

CrystalCap ALS (HR4-779) is supplied without a vial (HR4-904).

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS is compatible with ALS style grippers, auto mounters and sample handlers.

ALS style sample mounters accept the CrystalCap ALS.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A • Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

CrystalCap ALS

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS HT is compatible with ALS style grippers, auto mounters and sample handlers.

The CrystalCap ALS HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

Note: CrystalCap ALS HT with Mounted CryoLoops are sold without vials. Vials HR4-904 sold separately.

Color Coded Cap…………CryoLoop Size
Red…………………………….0.025-0.05 mm
Green…………………………….0.05-0.1 mm
Blue………………………………..0.2-0.3 mm
Blue/Red………………………….0.3-0.4 mm
Green/Red………………………..0.4-0.5 mm
Yellow/Green……………………..0.7-1.0 mm

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

CrystalCap ALS HT

CrystalCap ALS HT

CrystalCap ALS HT

CrystalCap ALS HT

CrystalCap ALS HT

The CrystalCap SPINE Vial is a 1.8 ml cryo vial featuring two small vents and is compatible with the CrystalCap SPINE HT. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The CrystalCap SPINE Vial features chamfered edges for enhanced cap positioning as well as a magnetic alloy bottom for stability. The HR8-114 CrystalCap SPINE Vial does have a magnet on the bottom of the vial.

The hollowed out design of the CrystalCap SPINE HT is compatible with SPINE style grippers, auto mounters and sample handlers.

The CrystalCap SPINE HT features a two dimensional (2D) alphanumeric 16 x 16 data matrix code on the underside of the cap. Each cap is also color coded for CryoLoop size.

CrystalCap SPINE without 2D code/printing (HR8-094) is the CrystalCap SPINE HT cap only, without 2D code, without color code, without white white background on bottom of cap and no alphanumeric side labeling; cap only, without Mounted CryoLoop.

Note: Caps with Mounted CryoLoops are sold without vials. Vials available separately.

Color Coded Cap…………CryoLoop Size
Red…………………………….0.025-0.05 mm
Green…………………………….0.05-0.1 mm
Yellow………………………………0.1-0.2 mm
Blue………………………………..0.2-0.3 mm
Blue/Red…………………………..0.3-0.4 mm
Green/Red………………………..0.4-0.5 mm
Yellow/Red………………………..0.5-0.7 mm
Yellow/Green……………………..0.7-1.0 mm

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 21-ID-D LS-CAT, APS 21-ID-E LS-CAT, APS 21-ID-F LS-CAT, APS 21-ID-G LS-CAT, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• The Brazilian Synchrotron Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• National Synchrotron Light Source II, Upton, New York NSLS-II 17-ID-1 AMX and NSLS-II 17-ID-2 FMX. NSLS-II 19-ID NYX

Europe
• Cerdanyola del Vallés, Spain ALBA XALOC
• Berlin Electron Storage Ring Company for Synchrotron Radiation, Berlin, Germany BESSY 14.1, BESSY 14.2, BESSY 14.3
• Diamond Light Source, Didcot, Oxfordshire, England DIAMOND I02, DIAMOND I03, DIAMOND I04, DIAMOND I04.1, DIAMOND I24
• Elettra Sincrotrone Trieste, Trieste, Italy ELETTRA 5.2R
• European Molecular Biology Laboratory, Hamburg, Germany EMBL/DESY P13, EMBL/DESY P14
• European Synchrotron Radiation Facility, Grenoble, France ESRF BM14, ESRF BM30A, ID30A-1/MASSIF-1, ID30A-3/MASSIF-3, ESRF ID23-1, ESRF ID23-2, ESRF ID29
• Max-Lab, Lund University, Sweden MAX II I911-2, MAX II I911-3
• Swiss Light Source at Paul Scherer Institut, Switzerland SLS X06DA-PXIII, SLS X06SA-PXI, SLS X10SA-PXII
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• Beijing Synchrotron Radiation Facility, Beijing, China BSRF 1W2B, BSRF 3W1A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT

CrystalCap SPINE HT Cap/Vial

CrystalCap SPINE without 2D code/printing

CrystalCap SPINE Vial

Cap Container empty

Magnetic Base – Strong

Magnetic Base – Medium

Seeding allows one to grow crystals in the metastable zone. Crystallization in this zone provides control, reproducibility and an improved likelihood of a successful crystallization experiment. Also, crystals can grow from seeds but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated to the metastable zone, one can use the seeds to grow larger single crystals. By controlling the number of seeds introduced into the drop, one can control the number of crystals grown. It is not practically possible to measure and know the number of seeds introduced to a drop, but by performing serial dilutions from a concentrated seed stock, one can control the number of crystals grown in the drop.

Using the Seed Bead kits, one can create crystal seed stock for subsequent seeding experiments. Crystals are placed in the microcentrifuge tube with the Seed Bead(s) and either vortexed or sonicated to generate a seed stock. Then by performing serial dilutions, one can control the number and size of crystals in the experiment.

The Seed Bead kit is useful for the preparation of seed stocks for automated and semi-automated microseeding (D’Arcy 2007, Harlos 2008).

Each Seed Bead Kit (HR2-320) contains 24 special microcentrifuge tubes, each tube containing a single 3mm PTFE Seed Bead.

Each Seed Bead Steel Kit (HR4-780) contains 24 special microcentrifuge tubes, each tube containing a six 1.6 mm Stainless Steel Seed Beads.

Each Seed Bead Ceramic Kit (HR4-781) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Zirconium Silicate Ceramic Seed Beads.

Each Seed Bead Glass Kit (HR4-782) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Glass Seed Beads.

Seed Bead Kit

Seed Bead Steel Kit

Seed Bead Ceramic Kit

Seed Bead Glass Kit

1. Stura, E.A., Wilson, I.A., Methods: A Companion to Methods in Enzymology (1990) 1, 38-49.

2. Stura, E.A., Wilson, I.A., “Seeding Techniques” in Crystallization of Nucleic Acids and Proteins: A Practical Approach. Oxford University Press (1992) 99-126.

3. A method to produce microseed stock for use in the crystallization of biological macromolecules Luft, J.R., DeTitta, G.T. Acta Cryst. (1999). D55, 988-993.

4. J.R. Luft and G.T. DeTitta, Methods in Enzymology (1997) 276, 110-131.

5. Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement. Watanabe et al. Acta Cryst. (2006). D62, 784-792.

6. Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase. K. Honbou, S. Yuzawa, N. N. Suzuki, Y. Fujioka, H. Sumimoto and F. Inagaki. Acta Cryst. (2006). F62, 1021-1023 (Used seed bead to optimize)

7. Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain. Yuji Kobayashi et al. Acta Cryst. (2007). F63, 950-952

8. An automated microseed matrix-screening method. Allan D′Arcy, Frederic Villard and May Marsh. Acta Cryst. (2007). D63, 550-554

9. The role of bias in crystallization conditions in automated microseeding. Edwin Pozharski et al. Acta Cryst. (2008). D64, 1222-1227.

10. Transmission electron microscopy for the evaluation and optimization of crystal growth. Hilary P. Stevenson & Guillermo Calero et al. Acta Cryst. (2016) D72, 603-615.

11. Microseed matrix screening for optimization in protein crystallization: what have we learned? D’Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70 (Pt 9):1117-26. doi: 10.1107/S2053230X14015507. Epub 2014 Aug 29.

Catalog number HR3-511 is a 1.88 inch (48 mm) wide, 3 mil tape on a 43.7 yard (40 M) roll with a 1.5 inch core and is supplied with a green dispenser / cutter.

Catalog number HR4-511 is a 1.88 inch wide, 3 mil tape on a 109.3 yard roll with a 3 inch core and no dispenser / cutter.

Two strips of the HR3-511 or HR4-511 will seal a Corning, Cryschem, CrystalClear Strip, Greiner, Intelliplate, Linbro, Swissci (MRC), VDX and VDXm plate.

Catalog number HR4-506 is a 3 inch wide, 3 mil tape on a 54.86 yard roll with a 3 inch core and no dispenser / cutter.

One strip of the HR4-506 will seal a Corning, Cryschem M, CrystalClear Strip, Greiner, Intelliplate, Swissci (MRC) and VDXm plate.

Crystal Clear Mini Sealing Tape HR4-508 is a 0.75 inch (19 mm) wide, 3 mil tape on a 650 inch (16.5 M) roll and is supplied with a clear dispenser / cutter. The tape is useful for resealing or patching wells after mounting crystals. The tape is also wide enough to seal a single row of a Cryschem S or Cryschem M plate.

See chart for tape and plate compatibility.

1.88 inch wide Crystal Clear Sealing Tape

1.88 inch wide Crystal Clear Sealing Tape

3 inch wide Crystal Clear Sealing Tape

0.75 inch wide Crystal Clear Mini Sealing Tape

#11 Replacement Blade – Extremely sharp point for fine angle cutting. Stainless steel. Sold in a package of five blades.

X-Acto® is a registered trademark of Elmer’s Products, Inc.

X-Acto Gripster Knife

Replacement #11 Blade, 5/pk

The LCP Glass Sandwich Set is a specially designed plate for either automated or manual setting of 96 Lipidic Cubic Phase matrix screening experiments. The plate can also be used for the bicelle method and batch method. The thin (< 2 mm high) plates have exquisite optical properties and are well suited for the detection of microcrystals and for birefringence-free imaging between crossed polarizers. The plates allow for in meso crystallization trials with 50 nanoliters protein/lipid mesophase and 1 microliter precipitant solution per trial.

The 96 well LCP Glass Sandwich Plate consists of a) 127.8 x 85.5 x 1 mm glass base plate with the footprint of an SBS-compliant plate, a 140 µm thick double sticky spacer with 96 punched out holes and b) a 0.2 mm thick glass coverslip. The double sticky spacer is already adhered to the base plate. A brown paper liner covers and protects the top of the double sticky spacer and base plate. The 0.2 mm thick glass coverslip fits over and seals the entire 96 well lower plate. Alternatively a series of twenty-four siliconized 18 x 18 mm No. 1 square cover slides (HR3-152) can be used to seal the entire 96 well lower plate. Each 18 x 18 mm No. 1 cover slide seals 4 wells. Space for a bar code is available at the left end of the plate. Each well can contain 50 nanoliters of cubic phase and 1 microliter of crystallization reagent.

18 x 18 mm No. 1 Siliconized Cover Slides are made from chemically resistant borosilicate glass D 263 M of the first hydrolytic class. The slides are colorless, clear, and suitable for fluorescence microscopy. The slides feature a super hydrophobic surface on both sides. The slides are No. thickness (0.13 ro 0.16 mm). The slides are supplied in a two compartment plastic box, 100 slides per box, 10 boxes per case, 1,000 slides total for HR3-152.

The Tungsten-carbide glass cutter can be used for cutting and removal of the upper coverglass that seals the LCP Sandwich Set. Sold separately.

LCP Sandwich Set

18 x 18 mm No. 1 Siliconized Cover Slides, Squares

1. Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins. Vadim Cherezov and Martin Caffrey. J. Appl. Cryst. (2003). 36, 1372-1377.

2. A robotic system for crystallizing membrane and soluble proteins in lipidic mesophases. Vadim Cherezov, Avinash Peddi, Lalitha Muthusubramaniam, Yuan F. Zheng and Martin Caffrey. Acta Cryst. (2004). D60, 1795-1807 DOI: 10.1107/S0907444904019109.

3. Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure. Faham, S. & Bowie, J. U. (2002). J. Mol. Biol. 316, 1-6.