Iron Assay Kit -Colorimetric-试剂盒货号：I291
Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒
Iron Assay Kit -Colorimetric-
商品信息
储存条件：0-5度保存
运输条件：室温
特点：
● 适合组织检测
● 配有标准品，可定量二价、三价铁含量
● 吸光度法
下载说明书
产品文献
选择规格：
50tests
现货
组织/细胞检测
试剂盒配套计算器（点击查看下载）
活动进行中
试剂盒内含
产品概述
原理
金属离子选择性
加标回收实验
实验例
参考文献
活动进行中
订购满5000元，200元礼品等你拿
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试剂盒内含
产品概述
铁是生物体内最重要的金属元素之一，生物体内铁元素的含量虽少，但有着重要的生理作用。近年来，随着铁死亡概念的提出，细胞内铁离子的代谢过程的研究逐渐成为了研究热点。同仁化学研究所开发的 Iron Assay Kit-Colorimetric-试剂盒 是一套操作简便的比色法铁离子检测试剂盒。与其他市面上的检测试剂盒 相比 ，可以在更短的时间内进行快速检测。通过比较组织裂解液中的铁离子探针 3-(2-Pyridyl)-5,6-bis(5-sulfo-2-furyl)-1,2,4-triazine, disodium salt的 吸光度与还原成二价铁离子的三价铁标准品的吸光度，即可确定组织样品中的二价铁含量。同时，还可以通过试剂盒中的还原剂将样品中的三价铁还原成二价铁，以确定总铁含量，进而计算出样品中三价铁的含量。
原理
金属离子选择性
加标回收实验
实验例
组织样品中的Fe2+和 Fe3+的检测
1. 称量 100 mg肝脏组织 。
2. 加入 1 ml冷 PBS，移液器吹打 20次， Vortex振荡 10 s 14,000 RPM离心 4 min，去除上清 。
3. 将样品转移至研钵，加入液氮充分研磨成粉末后，加入 1.3 ml Assay Buffer，回收至微管中。
4. 将含有样品的微管置于超声波水浴中 5 min。（为防止样品温度上升，每 1 min间隔置于冰浴上 20 s）。
5. 16,000 g离心 10 min，除去不溶物。
6. 取 1300 μl上清液，每管分别加入 400 μl上清液，制成二价铁样品、总铁样品、样品空白。
7. Standard管 中加入 Reducer solution。 （参考图 2 H管 30 μl、 G~C管 20 μl、 B管 40 μl、 A管 20 μl）。
二价品样品管中加入 20 μl Assay Buffer。
总铁样品管中加入 20 μl Reducer solution。
样品空白管中加入 20 μl Assay Buffer。
将所有的 Standard管、样品管、样品空白管 37 培养 15 min。
8. 参考表 1和表 2，加入至 96孔板中 37 培养 60 min，检测 593 nm处的吸光度。
9. 将酶标仪数据拷贝至 “Iron Assay Kit – Excel表 ”，自动计算 出二价铁和三价铁浓度。
参考文献
| No. | 检测对象 | 引用 (含链接) | 影响因子 |
| 1 | 组织
(小鼠肿瘤组织) |
H. Ren, J. Yong, Q. Yang, Z. Liu, Y. Xu, H. Wang, X. Jiang, W. Miao, X. Li, “Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy”, Acta Pharm. Sin. B, 2021, doi:10.1016/j.apsb.2021.05.005. | 7.097 |
| 2 | 细胞
Kasumi-1 (人急性白血病细胞) |
L. Zhang, Y. Song, K. Cao, Y. Du, M. Han, Z. Shi, F. Yan and S. Feng, “Hepcidin-Based Nanocomposites for Enhanced Cancer Immunotherapy by Modulating Iron Export-Mediated N6-Methyladenosine RNA Transcript”, Adv. Funct. Mater., 2021, doi: 10.1002/adfm.202107195. | 18.808 |
| 3 | 细胞
(II型肺泡细胞) |
H. Cheng, D. Feng, X. Li, L. Gao, S. Tang, W. Liu, X. Wu, S. Yue, C. Li, Z. Luo, “Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis”, Biochim. Biophys. Acta, Mol. Basis Dis., 2021, doi: 10.1016/j.bbadis.2021.166204. | 5.187 |
| 4 | 细胞
MV4-11; Kasumi-1; NB4 (白血病细胞) |
Y. Du, M. Han, K. Cao, Q. Li, J. Pang, L. Dou, S. Liu, Z. Shi, F. Yan and S. Feng, “Gold Nanorods Exhibit Intrinsic Therapeutic Activity via Controlling N6‑Methyladenosine Based Epitranscriptomics in Acute Myeloid Leukemia”, ACS Nano, 2021, doi: 10.1021/acsnano.1c05547. | 15.881 |
| 5 | 组织
(人软骨组织) |
H. Ren, J. Yong, Q. Yang, Z. Yang, Z. Liu, Y. Xu, H. Wang, X. Jiang, W. Miao, X. Li, “Contribution of ferroptosis and GPX4’s dual functions to osteoarthritis progression”, EBioMedicine, 2022, doi: 10.1016/j.ebiom.2022.103847. | 8.143 |
| 6 | 组织
(小鼠肺、肝脏、肾脏) |
T. Wu, X. Wang, M. Chen, X. Zhang, J. Zhang, J. Cheng, L. Kong, M. Tang,”Respiratory exposure to graphene quantum dots causes fibrotic effects on lung, liver and kidney of mice”, Food Chem. Toxicol., 2022, doi: 10.1016/j.fct.2022.112971. | 6.023 |
| 7 | 组织
(小鼠海马组织) |
T. Wu, X. Wang, J. Cheng, X. Liang, Y. Li, M. Chen, L. Kong and M. Tang, “Nitrogen‑doped graphene quantum dots induce ferroptosis through disrupting calcium homeostasis in microglia”, Part. Fibre Toxicol., 2022, doi: 10.1186/s12989-022-00464-z. | 9.4 |
| 8 | 组织
(宫颈管粘膜) |
T. Wang, M. Gong, Y. Cao, C. Zhao, Y. Lu, Y. Zhou, S. Yao, J. Chen, C. Zhao and R. Ju, “Persistent ferroptosis promotes cervical squamous intraepithelial lesion development and oncogenesis by regulating KRAS expression in patients with high risk-HPV infection”, 2022, Cell Death Discovery, doi: 10.1038/s41420-022-01013-5. | 5.241 |
| 9 | 组织
(小鼠肺组织) |
M. Li, L. Fu, B. Lv, Y. Xiang, H. Xiang, D. Xu, H. Zhao, “Arsenic induces ferroptosis and acute lung injury through mtROS-mediated mitochondria-associated endoplasmic reticulum membrane dysfunction”, Ecotoxicol. Environ. Saf., 2022, doi: 10.1016/j.ecoenv.2022.113595. | 6.291 |
| 10 | 细胞
BEL-7402, SMMC-7721 (人肝癌细胞系) |
L. Guan, F. Wang, M. Wang, S. Han, Z. Cui, S. Xi, H. Xu and S. Li, “Downregulation of HULC Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the miR-3200-5p/ATF4 Axis”, 2022, Oxid. Med. Cell. Longevity, doi: 10.1155/2022/9613095. | 6.543 |
| 11 | 组织
(小鼠肺部组织) |
Z. Zeng, H. Huang, J. Zhang, Y. Liu, W. Zhong, W. Chen, Y. Lu, Y. Qiao, H. Zhao, X. Meng, F. Zou, S. Cai, H. Dong, “HDM induce airway epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma”, FASEB J., 2022, doi: 10.1096/fj.202101977RR. | 5.191 |
| 12 | 组织
(大豆叶片) |
Y. Cheng, H. Zhang, W. Zhu, Q. Li, R. Meng, K. Yang, Z. Guo, Y. Zhai, R. Ji, H. Peng, D. Dou, M. Jing, “Ferroptosis induced by the biocontrol agent Pythium oligandrum enhances soybean resistance to Phytophthora sojae”, Environ. Microbiol., doi: 10.1111/1462-2920.16248. | 5.476 |
| 13 | 组织
(小鼠睾丸组织) |
Y. Zhao, H. Zhang, J. Cui, J. Wang, M. Chen, H. Wang, X. Li, J. Li, “Ferroptosis is critical for phthalates driving the blood-testis barrier dysfunction via targeting transferrin receptor”, Redox Biol., 2022, doi: 10.1016/j.redox.2022.102584. | 10.787 |
| 14 | 组织
(小鼠结肠组织) |
F. Yang, Y. Chen, Y. Xiao, H. Jiang, Z. Jiang, M. Yang, M. Li, Y. Su, Z. Yan, Y. Lin, D. Li, “pH-sensitive Molybdenum (Mo)-based polyoxometalate nanoclusters have therapeutic efficacy in Inflammatory Bowel disease by counteracting ferroptosis”, Pharmacol. Res., 2023, doi: 10.1016/j.phrs.2023.106645. | 10.334 |
| 15 | 组织
(小鼠肺部组织) |
W. Tang, J. Qin, Y. Zhou, W. Wang, F. Teng, J. Liu, L. Yi, J. Cui, X. Zhu, S. Wang, J. Dong, Y. Wei, “Regulation of ferroptosis and ACSL4-15LO1 pathway contributed to the anti-asthma effect of acupuncture”, Int. Immunopharmacol., 2023, doi: 10.1016/j.intimp.2022.109670. | 5.714 |
| 16 | 组织
(小鼠心脏组织) |
H. Hu, L. Li, H. Zhang, Y. Zhang, Q. Liu, M. Chen, J. Ning, Y. Pang, W. Hu, Y. Niu, R. Zhang, “Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis”, Chemosphere, 2023, doi: 10.1016/j.chemosphere.2023.137749. | 8.943 |
| 17 | 细胞
(HK-2, 人肾皮质近曲小管上皮细胞) |
L. Kang, D. Wang, T. Shen, X. Liu, B. Dai, D. Zhou, H. Shen, J. Gong, G. Li, Y. Hu, P. Wang, X. Mi, Y. Zhang, X. Tan, “PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma”, Cell Death Dis., 2023, doi: 10.1038/s41419-023-05719-x. | 9.685 |
| 18 | 细胞
(HK-2, 人肾皮质近曲小管上皮细胞) |
C. Dong, C. Song, Z. He, Q. Song, T. Song, J. Liu, Y. Xiong, X. Su, J. Zhou, X. Yang, W. Liao, “Protective efficacy of Schizandrin B on ameliorating nephrolithiasis via regulating GSK3β/Nrf2 signaling-mediated ferroptosis in vivo and in vitro”, 2023, Int. Immunopharmacol., doi:10.1016/j.intimp.2023.110042. | 5.714 |
| 19 | 组织
(小鼠肺部组织) |
B. Guo, Z. Zuo, X. Di, Y. Huang, G. Gong, B. Xu, L. Wang, X. Zhang, Z. Liang, Y. Hou, X. Liu, Z. Hu, “Salidroside attenuates HALI via IL-17A-mediated ferroptosis of alveolar epithelial cells by regulating Act1-TRAF6-p38 MAPK pathway”, 2022, Cell Commun. Signaling, doi: 10.1186/s12964-022-00994-1. | 7.525 |
| 20 | 组织
(小鼠睾丸组织) |
J. Ding, B. Lu, L. Liu, Z. Zhong, N. Wang, B. Li, W. Sheng, Q. He, “Guilu-Erxian-Glue alleviates Tripterygium wilfordii polyglycoside-induced oligoasthenospermia in rats by resisting ferroptosis via the Keap1/Nrf2/GPX4 signaling pathway”, Pharm. Biol., 2023, doi:10.1080/13880209.2023.2165114. | 3.889 |
| 21 | 细胞
(人髓核细胞) |
X. Yang, Y. Chen, J. Guo, J. Li, P. Zhang, H. Yang, K. Rong, T. Zhou, J. Fu, J. Zhao, “Polydopamine Nanoparticles Targeting Ferroptosis Mitigate Intervertebral Disc Degeneration Via Reactive Oxygen Species Depletion, Iron Ions Chelation, and GPX4 Ubiquitination Suppression”, Adv. Sci., 2023, doi:10.1002/advs.202207216. | 17.521 |
| 22 | 组织
(小鼠心脏组织) |
J. Pan, W Xiong, A. Zhang, H. Zhang, H. Lin, L. Gao, J. Ke, S. Huang, J. ZHang, J. Gu, A. Chang, C. Wang, “The Imbalance of p53–Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin-Challenged Cardiomyocytes”, Adv. Sci, 2023, doi:10.1002/advs.202206007. | 17.521 |
| 23 | 细胞
(H9c2, 4T1) |
T. Chen, Y. Qin, Y. Li, Y. Li, J. Luo, L. Fan, M. Feng, Z. Wang and Y. Zhao, “Chiral Polymer Micelles Alleviate Adriamycin Cardiotoxicity via Iron Chelation and Ferroptosis Inhibition”, Adv. Funct. Mater., 2023, doi:10.1002/adfm.202300689. | 19.923 |
| 24 | 细胞
(乳腺癌细胞) |
Z. ZHu, H. Shen, J. Xu, Z. Fang, G. Wo, Y. Ma, K. Yang, Y. Wang, Q. Yu, J. Tang., “GATA3 mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction in breast cancer cells”, Drug Resistance Updates, 2023, doi:10.1016/j.drup.2023.100974. | 22.841 |
| 25 | 组织
（the brains of 3 × Tg-AD mice） |
Xinlu Li, Jianfeng Chen, Wennuo Feng ,Chao Wang, Minyu Chen, Yifan Li, Jinghong Chen, Xinwei Liu, Qiong Liu, Jing Tian,“Berberine ameliorates iron levels and ferroptosis in the brain of 3 × Tg-AD mice”2023, PHYTOMEDICINE, doi:10.1016/j.phymed.2023.154962
|
7.9 |
| 26 | 组织
（Testicular tissues） |
Lipeng Li , Zijie Pei , Ruiting Wu , Yaling Zhang , Yaxian Pang , Huaifang Hu , Wentao Hu , Zihan Geng , Tengfei Feng , Yujie Niu , Guimin Hao , Rong Zhang“FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice”，Ecotoxicology and Environmental Safety，2023，doi.org/10.1016/j.ecoenv.2023.115309 | 6.8 |
| 27 | 细胞（macrophage） | Wenlin Yuan , Yuting Yang , Yingming Wei , Xufei Yu , Jiaqi Bao , Jiahui Zhong , Zhongxiu Wang , Lili Chen“Macrophage ferroptosis promotes MMP2/9 overexpression induced by hemin in hemorrhagic plaque”，Thromb Haemost，2023，doi.org/10.1016/j.intimp.2023.110916 | 6.7 |
| 28 | 细胞（HT-22） | Zixuan Yuan,ORCID,Xiaoming Zhou ,Yan Zou ,Bingtao Zhang ,Yao Jian ,Qi Wu ,Shujuan Chen and Xin Zhang“Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy”，Antioxidants，2023，doi.org/10.3390/antiox12122097 | 7.0 |