Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimization Screens > StockOptions Buffer Kits > StockOptions Sodium Acetate Buffer Kit

StockOptions Sodium Acetate Buffer Kit

应用

  • Focused, titrated buffer stocks for crystal screening, optimization, & production
  • 用于晶体筛选、优化和生产的集中滴定缓冲液

特征

  • 0.1 unit increments for fine tuning pH
  • pH 3.6 – 5.6
  • 1.0 M concentrated stocks
  • pKa = 4.8

描述

StockOptions Buffer kits are reagent toolboxes for the macromolecular crystal grower. Stock-Options reagent kits offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in StockOptions kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.

Each HR2-233 contains 21 unique reagents covering the pH range of 3.6 to 5.6 in 0.1 pH increments. Each reagent is supplied in a 10 milliliter volume and is titrated using HCl.

StockOptions 缓冲液试剂盒是大分子晶体生长器的试剂工具箱。 Stock-Options 试剂盒以方便、经济高效的试剂盒形式提供精确配制的高质量结晶级试剂库存。 StockOptions 套件中使用的化学品与 Hampton Research 套件中使用的结晶级超高纯度化学品相同。 StockOptions 试剂按照严格的质量标准精心配制,以确保可靠的性能和批次间的一致性。

预先配制的库存消除了计算、测量和配方错误。 无需再猜测试剂是如何配制的、使用了何种特定化学品、何时制造,或者在试剂消失后如何精确复制该试剂,并且需要更多试剂来进行额外设置。

每个 HR2-233 包含 21 种独特的试剂,涵盖 3.6 至 5.6 的 pH 范围,以 0.1 pH 为增量。 每种试剂的容量为 10 毫升,并使用 HCl 进行滴定。

Sodium acetate trihydrate
Synonym: Acetic acid sodium salt trihydrate
C2H3NaO2·3H2O
CH3COONa·3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity >99.5%
pKa = 4.8

Assay
Al <0.0005%
As <0.00001%
Bi <0.0005%
Ca <0.001%
Cd <0.0005%
Cl <0.0005%
Co <0.0005%
Cr <0.0005%
Cu <0.0005%
Fe <0.0005%
K <0.005%
Li <0.0005%
Mg <0.0005%
Mn <0.0005%
Mo <0.0005%
Ni <0.0005%
Pb <0.0005%
PO4 <0.0005%
SO4 <0.002%
Sr <0.0005%
Zn <0.0005%

HR2-233 StockOptions Sodium Acetate Buffer Kit 10 mL tube format

StockOptions Sodium Acetate Buffer Kit
StockOptions Sodium Acetate Buffer Kit
StockOptions Sodium Acetate Buffer Kit
StockOptions Sodium Acetate Buffer Kit
CAT NO

HR2-233

NAME

StockOptions Sodium Acetate Kit

DESCRIPTION

pH 3.6 to 5.6 in 0.1 pH unit increments

PRICE

$265.00

 

相关项目

  • Sodium acetate trihydrate Buffer
  • Individual StockOptions Sodium Acetate Reagents
  • 三水乙酸钠缓冲液
    个人股票期权乙酸钠试剂

参考

1. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens

2. Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer. Weichsel, A; Gasdaska, JR; Powis, G; Montfort, WR. Structure 4, 735- 751, 1996.

3. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.

1. 膜蛋白结构的微尺度荧光热稳定性分析,第 16 卷,第 3 期,2008 年 3 月 11 日,第 351-359 页 Alexander I. Alexandrov、Mauro Mileni、Ellen Y.T. Chien、迈克尔 A. 汉森、雷蒙德 C. 史蒂文斯

2. 还原、氧化和突变的人硫氧还蛋白的晶体结构:调节同源二聚体的证据。 魏克塞尔,一个; 加斯达斯卡,JR; 鲍伊斯,G; 蒙福特,WR。 结构 4, 735-751, 1996。

3. 通过对 pH 限值进行精细采样来增加微晶的尺寸。 A.麦克弗森。 J.应用。 水晶。 (1995 年)。 28, 362-365。

Hampton蛋白结晶试剂盒Acetic acid

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Acetic acid

Acetic acid

应用

  • Crystallization grade Acetic acid for altering drop and reservoir pH
  • 结晶级乙酸,用于改变液滴和储液器的 pH 值

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Acetic acid

Synonyms: Glacial acetic acid
CAS Number [64-19-7]
CH3CO2H
Mr 60.05
Beilstein Registry Number 506007
EC Number 200-580-7
MDL number MFCD00036152
PubChem Substance ID 24859247

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

别名:冰醋酸
CAS 编号 [64-19-7]
CH3CO2H
60.05 先生
贝尔斯坦注册号 506007
欧盟编号 200-580-7
MDL 编号 MFCD00036152
PubChem 物质 ID 24859247

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法,请参阅 pdf 使用挥发性缓冲液。

Acetic acid

Acetic acid
CAT NO

HR2-853

NAME

5.2 M Acetic acid

DESCRIPTION

15 mL

PRICE

$24.00

相关项目

  • Ammonium hydroxide
  • 氢氧化铵

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

3. Crystallization and preliminary X-ray diffraction studies of the engrailed homeodomain and of an engrailed homeodomain/DNA complex. Liu et al, Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 1990, pages 257-259.

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

7. The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue. Muranova et al, Acta Cryst. (2001). D57, 1192±1195

1. 蛋白质晶体的制备和分析。 Alexander McPherson,1982,John Wiley and Sons, Inc.,由 Krieger Publishing 印刷。第 4 章,结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森,欧元。 J.生化。 189, 1-23 (1990)。

3. engrailed homeodomain 和 engrailed homeodomain/DNA 复合物的结晶和初步 X 射线衍射研究。刘等人,生化和生物物理研究通讯,卷。 171,第 1 期,1990 年,第 257-259 页。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人,J. Appl。水晶。 (1989 年)。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。 (1989 年)。 22、115-118

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人,Acta Cryst。 (2003 年)。 D59,1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人,Acta Cryst。 (2001 年)。 D57, 1192±1195

Hampton蛋白结晶试剂盒Ammonium hydroxide

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Ammonium hydroxide

Ammonium hydroxide

应用

  • Crystallization grade Ammonium hydroxide for altering drop and reservoir pH
  • 用于改变液滴和油藏 pH 值的结晶级氢氧化铵

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium hydroxide

Synonyms: Ammonia aqueous, Ammonia water
CAS Number [1336-21-6]
NH4OH
Mr 35.05
Beilstein Registry Number 3587154
EC Number 215-647-6
MDL Number MFCD00066650

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

同义词:氨水、氨水
CAS 编号 [1336-21-6]
NH4OH
35.05 先生
贝尔斯坦注册号 3587154
欧盟编号 215-647-6
MDL 编号 MFCD00066650

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法,请参阅 pdf 使用挥发性缓冲液。

Ammonium hydroxide

Ammonium hydroxide
CAT NO

HR2-855

NAME

5.2 M Ammonium hydroxide

DESCRIPTION

10 mL

PRICE

$24.00

 

相关项目

  • Acetic acid 醋酸

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

3. Crystallization and preliminary X-ray diffraction studies of the engrailed homeodomain and of an engrailed homeodomain/DNA complex. Liu et al, Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 1990, pages 257-259.

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118.

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

7. The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue. Muranova et al, Acta Cryst. (2001). D57, 1192±1195.

1. 蛋白质晶体的制备和分析。 Alexander McPherson,1982,John Wiley and Sons, Inc.,由 Krieger Publishing 印刷。第 4 章,结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森,欧元。 J.生化。 189, 1-23 (1990)。

3. engrailed homeodomain 和 engrailed homeodomain/DNA 复合物的结晶和初步 X 射线衍射研究。刘等人,生化和生物物理研究通讯,卷。 171,第 1 期,1990 年,第 257-259 页。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人,J. Appl。水晶。 (1989 年)。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。 (1989 年)。 22, 115-118。

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人,Acta Cryst。 (2003 年)。 D59,1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人,Acta Cryst。 (2001 年)。 D57,1192±1195。

Hampton蛋白结晶试剂盒BICINE Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BICINE Buffer

BICINE Buffer

应用

  • Crystallization grade BICINE buffer for formulating screens or for optimization
  • 用于配制屏幕或优化的结晶级 BICINE 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BICINE

Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
Mr 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
PubChem Substance ID 24891738
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C

Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 – 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 – 167.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 – 1.35821 at 20°C

HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 – 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 – 1.36122 at 20°C

同义词: N,N-双(2-羟乙基)甘氨酸
C6H13NO4
163.17先生
CAS 编号 [150-25-4]
欧盟编号 205-755-1
贝尔斯坦注册号 1769362
默克 14,1201
RTECS MB9700000
MDL 编号 MFCD00004295
PubChem 物质 ID 24891738
纯度≥99.0%
pKa 在 20°C 时为 8.3,在 25°C 时为 8.3

有用的 pH 范围 7.4-9.3
使用 NaOH 将 HR2-509 滴定至有用的 pH 范围
HR2-509 pH 范围:25°C 时为 5.0 – 5.3
HR2-509 测量电导率范围:25°C 时为 100.7 – 167.2 µS/cm
HR2-509 测量的折射率范围:20°C 时为 1.35769 – 1.35821

HR2-723 已使用 NaOH 滴定至 pH 9.0
HR2-723 测得的电导率范围:23.9 – 25.0 mS/cm,25°C
HR2-723 测量的折射率范围:20°C 时为 1.36054 – 1.36122

BICINE

BICINE Buffer
CAT NO

HR2-509

NAME

1.0 M BICINE

DESCRIPTION

100 mL

PRICE

$104.00

CAT NO

HR2-723

NAME

1.0 M BICINE pH 9.0

DESCRIPTION

100 mL

PRICE

$104.00

相关项目

  • Sodium hydroxide
  • Individual StockOptions Bicine Reagents
  • StockOptions Bicine Buffer Kit
  • 氢氧化钠
    个别股票期权 Bicine 试剂
    StockOptions Bicine 缓冲套件

参考

1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.

1. 来自 Ophiostoma novo-ulmi 的真菌水解酶的结晶和初步 X 射线衍射研究。 M. N. Isupov、A. A. Brindley、E. J. Hollingsworth、G. N. Murshudov、A. A. Vagin 和 J. A. Littlechild。 晶体学报。 (2004 年)。 D60,1879-1882。

Hampton蛋白结晶试剂盒BIS-TRIS Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer

BIS-TRIS Buffer

应用

  • Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 BIS-TRIS 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS

Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C

HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C

HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C

同义词:2,2-双(羟甲基)-2,2′,2″”-次氮基三乙醇,或 2-双(2-羟乙基)氨基-2-(羟甲基)-1,3-丙二醇,或双(2- 羟乙基)氨基-三(羟甲基)甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C

使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围:25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率:20°C 时为 1.37069 – 1.37150

HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围:22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率:20°C 时为 1.36817 – 1.36926

Al  ≤0.0005%
Ca ≤0.0005%
Cl  ≤0.05%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na  ≤0.005%
Pb  ≤0.001%
SO4 ≤0.05%
Zn  ≤0.0005%

BIS-TRIS

BIS-TRIS Buffer
CAT NO

HR2-781

NAME

1.0 M BIS-TRIS pH 5.5

DESCRIPTION

100 mL

PRICE

$169.00

CAT NO

HR2-783

NAME

1.0 M BIS-TRIS pH 6.5

DESCRIPTION

100 mL

PRICE

$169.00

相关项目

  • Hydrochloric acid
  • StockOptions Bis-Tris Buffer Kit
  • Individual StockOptions Bis-Tris Reagents
  • 盐酸
    StockOptions Bis-Tris 缓冲套件
    个别股票期权 Bis-Tris 试剂

参考

1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.

2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.

1. 噬菌体 G4 降解前衣壳颗粒的原子结构:在钙离子存在下诱导结构变化和功能影响。 麦肯纳,R; 鲍曼,BR; 伊拉格,LL; 罗斯曼,MG; 法恩,文学士。 分子生物学杂志 256, 736-750, 1996。

2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。 蒙托亚,G; 斯文森,C; 卢林克,J; Sinning, I. 蛋白质 28, 285-288, 1997。

Hampton蛋白结晶试剂盒Parabar 10312 (previously known as Paratone)

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

应用

  • Oil based cryoprotectant

特征

  • Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
  • Oil based cryoprotectant

描述

Sorry, Parabar 10312 is no longer available.  We’ve left this page up for reference only.  See HR2-861 Santovac Cryo Oil as a possible substitute.

抱歉,Parabar 10312 不再可用。 我们保留此页面仅供参考。 请参阅 HR2-861 Santovac Cryo Oil 作为可能的替代品。

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512.

Paratone® is a registered trademark of Chevron Corporation.

Parabar 10312 是一种粘性冷冻保护剂,适用于小分子和大分子晶体学。 1

可以在惰性气氛下用 Parabar 包覆对空气轻微不稳定的小分子化合物。 Parabar 保护的晶体样品可以在冷冻氮气流中进行低温冷却。 Parabar 也已成功用作生物大分子晶体的冷冻保护剂。在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用 Parabar 涂覆晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)向晶体的暴露和缓慢扩散。

HR2-643 Parabar 10312 不再可用,自 2021 年 5 月 11 日起停产。

HR2-862 Parabar 10312 以 5 ml 装在琥珀色玻璃小瓶中,螺旋盖盖中带有玻璃涂抹棒。

通过将 50:50 与石蜡油混合,可以降低 Parabar 的粘度,以改善易碎针状和刀片状晶体的处理。

Parabar 是聚异丁烯的混合物,Staudinger 分子量约为 20,000,含有约 20% 的聚合物。

20°C 时的折射率为 1.48487。

100°C 时运动粘度为 641 cSt。 100°C 时运动粘度比为 2.05。

清晰到略微朦胧的是外观。

晶体学文献中使用的同义词:Paratone® N、Paratone® 8277、润英联 V8512。

Paratone® 是雪佛龙公司的注册商标。

Parabar 10312

Parabar 10312 (previously known as Paratone)
Parabar 10312 (previously known as Paratone)

相关项目

  • Perfluoropolyether Cryo Oil
  • Santovac Cryo Oil
  • 全氟聚醚冷冻油
    Santovac 冷冻油

参考

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.

1. H. Hope,生物大分子的低温晶体学:一种普遍适用的方法。 晶体学报。 (1988) B44,22-26。

2. 与选择性调节剂 LGD2226 复合的人雄激素受体的配体结合域 (LBD) 的结构。 F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi 和 T. Jiang。 晶体学报。 (2006 年)。 F62, 1067-1071。

3. H.霍普,安努。 牧师生物物理学。 化学。 1990 年 19:107-126。

4. S. Parkin 和 H. Hope,J. Appl。 水晶。 (1998) 第 945-953 页。

Hampton蛋白结晶试剂盒BIS-TRIS propane Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS propane Buffer

BIS-TRIS propane Buffer

应用

  • Crystallization grade BIS-TRIS propane pH 7.0 for formulating screens or for optimization
  • 结晶级 BIS-TRIS 丙烷 pH 7.0,用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS propane

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
Mr 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C

HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C

别名:1,3-双[三(羟甲基)甲氨基]丙烷
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
282.34 先生
CAS 编号 [64431-96-5]
欧盟编号 264-899-3
贝尔斯坦注册号 1786109
MDL 编号 MFCD00004689
PubChem 物质 ID 24891934
纯度≥99.0%
pKa1 = 6.8 在 25°C
pKa2 = 9.0 在 25°C

HR2-795 使用 HCl 滴定至 pH 7.0
测得的电导率范围:25°C 时 41.9 – 43.5 mS/cm
测得的折射率范围:20°C 时为 1.39110 – 1.39222

BIS-TRIS propane

BIS-TRIS propane Buffer
CAT NO

HR2-795

NAME

1.0 M BIS-TRIS propane pH 7.0

DESCRIPTION

100 mL

PRICE

$108.00

相关项目

  • Individual StockOptions Bis-Tris Propane Reagents
  • Citric acid BIS-TRIS propane Buffer
  • 个别股票期权 Bis-Tris 丙烷试剂
    柠檬酸 BIS-TRIS 丙烷缓冲液

参考

1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.

2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.

1. 结晶 DNA-EcoRI 核酸内切酶识别复合物的双重对称性。 格拉布尔,J; 加利福尼亚州弗雷德里克; 萨穆兹,C; 仁-雅各布森,L; 较小,D; 格林,P; 博耶,HW; 板仓,K; 罗森伯格,JM。 J Biomol Struct Dyn 1, 1149-1160, 1984。

2. 蛋白质折叠和稳定性中的非天然局部相互作用:在 AII β-片 α-Spectrin SH3 域中引入螺旋趋势。 普列托,J; 威尔曼斯,M; 马萨诸塞州希门尼斯; 里科,M; Serrano, L. J Mol Biol 268, 760-778, 1997。

Hampton蛋白结晶试剂盒Citric acid Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Citric acid Buffer

Citric acid Buffer

应用

  • Crystallization grade Citric acid buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级柠檬酸缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Citric acid

Synonyms: Citric acid anhydrous
C6H8O7
HOC(COOH)(CH2COOH)2
Mr 192.13
CAS Number [77-92-9]
EC Number 201-069-1
Beilstein Registry Number 782061
Merck 14,2326
RTECS GE7350000
MDL Number MFCD00011669
PubChem Substance ID 24856611
Purity ≥ 99.5%
Useful pH range of citric acid as a buffer 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 is titrated to pH 3.5 at 25 degrees Celsius using NaOH
Measured Conductivity Range: 28.7 – 33.3 mS/cm at 25°C
Measured Refractive Index Range: 1.36236 – 1.36329 at 20°C

别名: 无水柠檬酸
C6H8O7
HOC(COOH)(CH2COOH)2
192.13 先生
CAS 编号 [77-92-9]
欧盟编号 201-069-1
贝尔斯坦注册号 782061
默克 14,2326
RTECS GE7350000
MDL 编号 MFCD00011669
PubChem 物质 ID 24856611
纯度≥99.5%
柠檬酸作为缓冲液的有用 pH 值范围 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 在 25 摄氏度下使用 NaOH 滴定至 pH 3.5
测得的电导率范围:25°C 时 28.7 – 33.3 mS/cm
测得的折射率范围:20°C 时为 1.36236 – 1.36329

Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Oxalate (C2O4) ≤0.05%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr ≤0.0005%
Tartrate (C4H4O6) ≤0.2%
Zn ≤0.0005%

Citric acid

Citric acid Buffer
CAT NO

HR2-757

NAME

1.0 M Citric acid pH 3.5

DESCRIPTION

100 mL

PRICE

$44.00

相关项目

  • Sodium hydroxide
  • StockOptions Citric Acid Buffer Kit
  • Individual StockOptions Citric Acid Reagents
  • 氢氧化钠
    StockOptions 柠檬酸缓冲液套件
    个别股票期权柠檬酸试剂

参考

1. Crystallization and preliminary X-ray structure solution of Rhizomucor miehei aspartic proteinase. Jia, Z; Vandonselaar, M; Schneider, P; Quail, JW. Acta Crystallogr D 51, 243- 244, 1995.

1.米黑根毛霉天冬氨酸蛋白酶的结晶和初步X射线结构解。 贾,Z; 范登塞拉,M; 施耐德,P; 鹌鹑,JW。 晶体学学报 D 51, 243-244, 1995。

Hampton蛋白结晶试剂盒Citric acid BIS-TRIS propane Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Citric acid BIS-TRIS propane Buffer

Citric acid BIS-TRIS propane Buffer

应用

  • Crystallization grade CBTP buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 CBTP 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Citrate BIS-TRIS propane (CBTP) is a buffer system useful across pH 2.5 to 9.5.

Using CBTP one can use a single buffer system to screen pH 2.5 to 9.5 by varying the ratio of Citric acid to BIS-TRIS propane. Although the two buffer reagents are made available separately for convenient, individual replacement, they are designed to be used together, with the supplied CBTP buffer titration table to create a crystallization buffer system covering pH 2.5 to 9.5.

HR2-831 Measured pH range: 1.2 – 1.5 at 25°C
HR2-831 Measured Conductivity Range: 7.7 – 8.7 mS/cm at 25°C

HR2-833 Measured pH range: 11.2 – 11.5 at 25°C
HR2-833 Measured Conductivity Range: 298 – 317 µS/cm at 25°C
HR2-833 Measured Refractive Index Range: 1.38000 – 1.38076 at 20°C

柠檬酸盐 BIS-TRIS 丙烷 (CBTP) 是一种适用于 pH 2.5 至 9.5 的缓冲系统。

使用 CBTP,可以使用单一缓冲系统通过改变柠檬酸与 BIS-TRIS 丙烷的比例来筛选 pH 2.5 至 9.5。 虽然这两种缓冲试剂是分开提供的,以便于单独更换,但它们被设计为一起使用,与提供的 CBTP 缓冲液滴定表一起创建一个涵盖 pH 2.5 至 9.5 的结晶缓冲系统。

HR2-831 测得的 pH 值范围:25°C 时为 1.2 – 1.5
HR2-831 测量电导率范围:25°C 时 7.7 – 8.7 mS/cm

HR2-833 测得的 pH 范围:25°C 时为 11.2 – 11.5
HR2-833 测量的电导率范围:298 – 317 µS/cm,25°C
HR2-833 测量的折射率范围:20°C 时为 1.38000 – 1.38076

Citric acid

Citric acid BIS-TRIS propane Buffer
Citric acid BIS-TRIS propane Buffer
CAT NO

HR2-831

NAME

1.0 M Citric acid

DESCRIPTION

100 mL

PRICE

$45.00

CAT NO

HR2-833

NAME

1.0 M BIS-TRIS propane

DESCRIPTION

100 mL

PRICE

$162.00

Hampton蛋白结晶试剂盒HEPES Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > HEPES Buffer

HEPES Buffer

应用

  • Crystallization grade HEPES buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 HEPES 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 HEPES

Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14,4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity ≥ 99.5%
pKa 7.5 at 25°C

Useful pH range of HEPES buffer 6.8 – 8.2

别名:2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic 酸)
C8H18N2O4S
238.31 先生
CAS 编号 [7365-45-9]
欧盟编号 230-907-9
贝尔斯坦注册号 883043
默克 14,4654
RTECS TL6809000
MDL 编号 MFCD00006158
PubChem 物质 ID 24895616
纯度≥99.5%
pKa 7.5 在 25°C

HEPES 缓冲液的有用 pH 范围 6.8 – 8.2

Titrate HR2-585 to the desired pH between 6.8 – 8.2 using NaOH
HR2-585 Measured pH Range: 5.4 – 5.5 at 25°C
HR2-585 Measured Conductivity Range: 160.1 – 236.9 μS/cm at 25°C
HR2-585 Measured Refractive Index Range: 1.37093 – 1.37131 at 20°C

HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 – 7.0 mS/cm at 25°C
HR2-785 Measured Refractive Index Range: 1.37133 – 1.37176 at 20°C

HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 – 13.6 mS/cm at 25°C
HR2-729 Measured Refractive Index Range: 1.37177 – 1.37290 at 20°C

Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO4 0.005% max

HEPES Buffer
CAT NO

HR2-585

NAME

1.0 M HEPES

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-785

NAME

1.0 M HEPES pH 7.0

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-729

NAME

1.0 M HEPES pH 7.5

DESCRIPTION

100 mL

PRICE

$113.00

相关项目

  • Sodium hydroxide
  • StockOptions Hepes Buffer Kit
  • Individual StockOptions HEPES Reagents
  • 氢氧化钠
    StockOptions Hepes 缓冲液套件
    个别股票期权 HEPES 试剂

参考

1. Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995.

1. 来自北大西洋鲑鱼 (Salmo salar) 的天然弹性蛋白酶的结晶和初步 X 射线晶体学研究。 伯格伦德,GI; 斯纳拉斯,AO; 汉森,LK。 晶体学学报 D 51, 393-394, 1995。