分类目录归档：Optimize – Solubilizing Agents (NDSB)

Hampton蛋白结晶试剂盒NDSB-195/HR2-703

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195

NDSB-195

NDSB-195
CAT NO

HR2-703

NAME

NDSB-195

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

NDSB-195 HR2-703 NDSB-195 User GuideNDSB-195 HR2-703 NDSB-195 SDS

应用

  • Crystallization grade NDSB-195 for formulating screens or for optimization
  • 结晶级 NDSB-195 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C7H17NO3S
Mr 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。

蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。

NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存

HR2-703

参考

1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.

2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.

4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

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上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

NDSB-201
CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

NDSB-201 HR2-701 NDSB-201 User GuideNDSB-201 HR2-701 NDSB-201 SDS

应用

  • Crystallization grade NDSB-201 for formulating screens or for optimization
  • 结晶级 NDSB-201 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C8H11NO3S
Mr 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.

Hampton蛋白结晶试剂盒NDSB-211/HR2-793

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上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211

NDSB-211

NDSB-211
CAT NO

HR2-793

NAME

NDSB-211

DESCRIPTION

5 grams

PRICE

$127.00

应用

  • Crystallization grade NDSB-211 for formulating screens or for optimization
  • 结晶级 NDSB-211 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C7H17NO4S
Mr 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

支持材料HR2-793

NDSB-211 HR2-793 NDSB-211 User GuideNDSB-211 HR2-793 NDSB-211 SDS

参考

1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.

Hampton蛋白结晶试剂盒NDSB-221/HR2-791

发表于由

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-221

NDSB-221

NDSB-221
CAT NO

HR2-791

NAME

NDSB-221

DESCRIPTION

5 grams

PRICE

$58.00

支持材料

NDSB-221 HR2-791 NDSB-221 User GuideNDSB-221 HR2-791 NDSB-221 SDS

应用

  • Crystallization grade NDSB-221 for formulating screens or for optimization
  • 结晶级 NDSB-221 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-221
Synonyms: Non-detergent sulfobetaine 221 or 3-(1-Methylpiperidinium)-1-propane Sulfonate
C9H19NO3S
Mr 221.34
Purity: ≥99.0%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-791参考

1. Goldberg, M.E., et al. 1996. Folding & Design 1, 21.

2. Protein crystallography with non detergent sulfobetaines. L. Vuillard et al. J Cryst Growth (1996) 168 150-15.

3. Vuillard L, Rabilloud T, Goldberg ME, Eur J Biochem 1998 256(1):128-135. (protein folding).

Hampton蛋白结晶试剂盒NDSB-256/HR2-705

发表于由

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256

NDSB-256

NDSB-256
CAT NO

HR2-705

NAME

NDSB-256

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

NDSB-256 HR2-705 NDSB-256 User GuideNDSB-256 HR2-705 NDSB-256 SDS

应用

  • Crystallization grade NDSB-256 for formulating screens or for optimization
  • 结晶级 NDSB-256 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256
Synonyms: Dimethylbenzylammonium propane sulfonate
C12H19NO3S
Mr 257.37
Purity: ≥99% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-705

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. A nondetergent sulfobetaine prevents protein aggregation in microcalorimetric studies. Collins et al, Notes & Tips / Anal. Biochem. 352 (2006) 299-301.

Hampton蛋白结晶试剂盒NDSB-256-4T/HR2-794

发表于由

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256-4T

NDSB-256-4T

NDSB-256-4T
CAT NO

HR2-794

NAME

NDSB-256-4T

DESCRIPTION

5 grams

PRICE

$172.00

支持材料

NDSB-256-4T HR2-794 NDSB-256-4T User GuideNDSB-256-4T HR2-794 NDSB-256-4T SDS

应用

  • Crystallization grade NDSB-256-4T for formulating screens or for optimization
  • 结晶级 NDSB-256-4T 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • NDSB 256 4T is a non-detergent sulfobetaine (NDSB) that prevents protein aggregation and facilitates protein folding by interacting with the early folding intermediates. Reported to improve the in vitro renaturation efficiency of reduced hen egg lysozyme (60% enzymatic activity at 600 mM NDSB-256-4T) and chemically unfolded tryptophan synthase ß2 subunit (100% enzymatic activity at 1 M NDSB-256-4T).
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂
    NDSB 256 4T 是一种非去污剂磺基甜菜碱 (NDSB)，可通过与早期折叠中间体相互作用来防止蛋白质聚集并促进蛋白质折叠。 据报道可提高还原鸡蛋溶菌酶（在 600 mM NDSB-256-4T 下的酶活性为 60%）和化学展开的色氨酸合酶 ß2 亚基（在 1 M NDSB-256-4T 下的酶活性为 100%）的体外复性效率。

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256-4T
Synonym: 3-(4-tert-Butyl-1-pyridino)-1-propane sulfonate, SB256-4T, SN2564T
C12H19NO3S
CAS Number [570412-84-9]
Mr 257.35
Purity = 98% by HPLC
Hygroscopic
Store at room temperature

参考NDSB-256-4T

1. Expert-Bezancon, N., et. al., Biophys Chem, 100, 469 (2003).