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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195
NDSB-195
CAT NO
HR2-703
NAME
DESCRIPTION
5 grams
PRICE
$57.00
支持材料
HR2-703 NDSB-195 User Guide
HR2-703 NDSB-195 SDS应用
- Crystallization grade NDSB-195 for formulating screens or for optimization
- 结晶级 NDSB-195 用于配制筛选或优化
特征
- Efficiently solubilizes proteins at non-denaturing concentrations
- Useful in preventing protein aggregation
- Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
- Protein specific additive for solubility and stability
- 在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂
描述
The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.
Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.
The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.
Typical useful NDSB concentration in protein sample is 0.5-1.0 M.
NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C7H17NO3S
Mr 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)
NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。
非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。
非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。
蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。
NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存
HR2-703
参考
1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.
4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.
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