衰老

细胞凋亡、细胞坏死及细胞自噬具有控制细胞生存及死亡的功能,对了解细胞的各种机能非常重要。
SA-β-gal
DNA损伤
核仁
品名 货号 用途
细胞衰老检测试剂盒—Cellular Senescence Detection Kit – SPiDER-βGal SG03 细胞衰老检测
SPiDER-βGal试剂 SG02 组织衰老检测
Cellular Senescence Plate Assay Kit – SPiDER-βGal试剂盒 SG05 细胞衰老检测(荧光酶标仪)

细胞衰老的特征

近几年发现了细胞衰老及具有致癌因子的衰老相关分泌表型(SASP)。在干细胞等领域均发现有衰老现象,在各领域的衰老研究受到越来越多的重视。

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通过不同指标评估衰老细胞

在不同传代数的WI-38细胞中,使用不同的试剂盒分别评估细胞的SA-ß-Gal活性,线粒体膜电位和细胞代谢情况(葡萄糖和乳酸)。在衰老细胞中,SA-ß-Gal活性增强,线粒体膜电位降低。其上清液中葡萄糖和乳酸的消耗量等代谢指标有所增加。

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细胞老化导致相关因素的变化

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引用论文

① H. Tanaka, S. Takebayashi, A. Sakamoto, N. Saitoh, S. Hino and M. Nakao, “The SETD8/PR-Set7 Methyltransferase Functions as a Barrier to Prevent Senescence-Associated Metabolic Remodeling.”Cell Reports, 2017, 18(9), 2148.
② L. Garcia-Prat, M. Martinez-Vicente and P. Munoz-Canoves, “Autophagy: a decisive process for stemness”Oncotarget, 2016, 7(11), 12286.
③ M. Bitar, S. Abdel-Halim and F. Al-Mulla, “Caveolin-1/PTRF upregulation constitutes a mechanism for mediating p53-induced cellular senescence: implications for evidence-based therapy of delayed wound healing in diabetes”Am J Physiol Endocrinol Metab., 2013, 305(8), E951.
④ C. Wiley, M. Velarde, P. Lecot, A. Gerencser, E. Verdin, J. Campisi, et. al., “Mitochondrial Dysfunction Induces Senescence with a Distinct Secretory Phenotype”Cell Metab., 2016, 23(2), 303.
⑤ E. Liao, Y. Hsu, Q. Chuah, Y. Lee, J. Hu, T. Huang, P-M Yang & S-J Chiu, “Radiation induces senescence and a bystander effect through metabolic alterations.”Cell Death Dis., 2014, 5, e1255.
⑥ K. Nishimura, T. Kumazawa, T. Kuroda, A. Murayama, J. Yanagisawa and K. Kimura, “Perturbation of Ribosome Biogenesis Drives Cells into Senescence through 5S RNP-Mediated p53 Activation”Cell Rep. 2015, 10(8), 1310.
⑦ M. J. Son, Y. Kwon, T. Son and Y. S. Cho, “Restoration of Mitochondrial NAD+ Levels Delays Stem Cell Senescence and Facilitates Reprogramming of Aged Somatic Cells”Stem Cells. 2016, 34(12), 2840.

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