细胞活性/毒性双重检测试剂盒——Viability/Cytotoxicity Multiplex Assay Kit货号：CK17

特色

发表于2024年7月2日由上海金畔生物科技有限公司

产品中心细胞增殖/毒性细胞活性/毒性双重检测试剂盒——Viability/Cytotoxicity Multiplex Assay Kit

细胞活性/毒性双重检测试剂盒——Viability/Cytotoxicity Multiplex Assay Kit货号：CK17

细胞活性/毒性双重检测试剂盒

Viability/Cytotoxicity Multiplex Assay Kit

商品信息

储存条件：0-5度保存

运输条件：室温

特点：

● CCK-8与LDH双指标检测毒性，数据更全面。

● 准备1次细胞，同时检测2项数据。

● 内含500T的CCK-8与LDH检测试剂盒，

价格优于单独购买。

下载说明书

SDS下载

选择规格：

500 tests

现货

双重实验同时检测

性价比高

CCK-8&LDH

更高灵敏度检测方法CCK-L（点击查看）

活动进行中

试剂盒内含

产品概述

产品特点

检测原理

同时检测的操作方法

性价比高

参考文献

活动进行中

订购满5000元，200元礼品等你拿

凑单关联产品TOP5

NO.1. ROS Assay Kit -Highly Sensitive DCFH-DA- ROS检测

NO.2. Annexin V, FITC Apoptosis Detection Kit 细胞凋亡检测

NO.3. Calcein-AM/PI Double Staining Kit 活死细胞双染检测

NO.4. Cell Cycle Assay Kit 细胞周期检测

NO.5. Caspase-3 Assay Kit-Colorimetric- 细胞凋亡检测

试剂盒内含

产品概述

细胞毒性检测通常通过检测活细胞数量或者死细胞数量来确定。而为了更加全面的评价细胞毒性情况，多种评价方法相互验证的需求与日俱增。Viability/CytotoxicityMultiplex Assay Kit可以综合评价细胞毒性。试剂盒内含Cell Counting Kit-8 (CCK-8) 和Cytotoxicity LDH Assay Kit-WST^®(LDH Assay Kit)，分别用于测定活细胞和死细胞数量。

CCK-8通过检测活细胞中脱氢酶活性来确定活细胞数，而LDH Assay Kit通过检测受损细胞膜释放LDH的量来确定死细胞数。LDH Assay Kit可以直接添加到含有细胞的培养基中检测 (一步法)。也可以分离细胞后，加到细胞培养基上清液中检测 (低损伤法，分离的细胞可以做其它实验)。两种细胞毒性评价指标，CCK-8和LDH Assay Kit(低损伤法) 可以使用同一批细胞同时进行实验。

产品特点

不同细胞毒性指标，双重印证。增加结果可信性。

单独检测CCK-8，细胞毒性判断不准确	2种指标一起检测，增加数据准确性
1、单纯检测活细胞数量（CCK-8、MTT、MTS、XTT等）	1、单纯检测活细胞数量（CCK-8、MTT、MTS、XTT等） 2、活细胞检测+死细胞检测（细胞膜损伤）

检测原理

LDH Assay Kit：细胞膜损伤，LDH外漏，通过检测LDH活性判断细胞毒性。

CCK-8：可以使用NADH作为指标来确认活细胞中的代谢活性。

同时检测的操作方法

本试剂盒包括用于测量活细胞的CCK-8和用于测量死细胞的LDH分析试剂盒，两者均可通过平板分析法在相同的吸收波长下进行评估。此外，同一细胞样本可用于同时评估活细胞和死细胞，从而节省了时间和精力。

性价比高

本产品作为CCK-8（500 tests）与Cytotoxicity LDH Assay Kit（500 tests）组合套装，低于单独购买的总价。适合新接触细胞毒性检测的用户，用于前期摸索实验条件。单独产品链接见下方。大包装购买，单价会降低很多。

Cell Counting Kit-8

Cytotoxicity LDH Assay Kit-WST

参考文献

1. Integrated Omics Reveals Tollip as an Regulator and Therapeutic Target for Hepatic Ischemia-Reperfusion Injury in Mice,

Hepatology., 2019, doi: 10.1002/hep.30705

2. Amplified Cancer Immunotherapy of Surface Engineering Antigenic Microparticles Vaccine by Synergistically Modulating Tumor

Microenvironment, ACS Nano, 2019, 13, 11, 12553-12566

3. A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head

and neck squamous cell carcinoma, Molecular Cancer, 2020,19, 4, doi: 10.1186/s12943-019-1123-y

4. Untangling the co-effects of oriented nanotopography and sustained anticoagulation in a biomimetic intima on neovessel

remodeling, Biomaterials, 2019, 119654

5. In Vitro and in Vivo Analysis of Mineralized Collagen-Based Sponges Prepared by a Plasma- and Precursor-Assisted

Biomimetic Process, ACS Applied Materials & Interfaces, 2017, 9, 27, 22185-22194

6. MoS2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with

endothelial cells on a microfluidic system, Biosensors and Bioelectronics, 2018, 99, 142-149

7. Biocompatible carbon nanotube fiber for implantable supercapacitors, Carbon, 2017, 122, 162-167

8. Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell

lung cancer, Cancer Letters, 2019, 442, 53-67

9. Knockdown of TGF-β1 expression in human umbilical cord mesenchymal stem cells reverts their exosome-mediated EMT

promoting effect on lung cancer cells, Cancer Letters, 2018, 428:34-44

10. Restriction of exogenous DNA expression by SAMHD1, Science Bulletin, 2020, https://doi.org/10.1016/j.scib.2019.12.028

11. PARP1 inhibition alleviates injury in ARH3-deficient mice and human cells, JCI Insight, 2019, 4(4), e124519

12. Downregulation of MCL-1 and upregulation of PUMA using mTOR inhibitors enhance antitumor efficacy of BH3 mimetics in

triple-negative breast cancer, Cell Death & Disease, 2018, 9(2), 137

13. Ischemic preconditioning attenuates ischemia/reperfusion-induced kidney injury by activating autophagy via the SGK1

signaling pathway, Cell Death & Disease, 2018, 9, 338

14. Pathological Endogenous a-Synuclein Accumulation in Oligodendrocyte Precursor Cells Potentially Induces Inclusions in

Multiple System Atrophy, Stem Cell Reports, 2018, 10(2), 356-365

15. PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration

in mice, FASEB Journal, 2019, 33(7), 8094-8109

16. Benzo[a]pyrene-decreased gap junctional intercellular communication via calcium/calmodulin signaling increases apoptosis in

TM4 cells, Journal of Applied Toxicology, 2018, 38(8), 1091-1103

关联产品

Cell Counting Kit-8细胞增殖毒性检测试剂盒CCK-8(CCK8)

细胞增殖-毒性检测试剂盒CCK-8(CCK8)

LDH检测试剂盒—Cytotoxicity LDH Assay Kit-WST

乳酸脱氢酶(LDH)检测试剂盒

Cell Counting Kit-Luminescence试剂盒

细胞增殖/毒性检测试剂盒-发光法(CCK-L)

Cell Count Normalization Kit试剂盒

细胞计数标准化试剂盒

Stop Solution for CCK-8试剂

CCK-8反应终止液

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

Hampton蛋白结晶试剂盒LCP Lipidic Cubic Phase Screening

发表于2024年7月27日由上海金畔生物科技有限公司

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Swissci > LCP Lipidic Cubic Phase Screening

LCP Lipidic Cubic Phase Screening

Applications

Lipidic cubic phase crystallization

Features

UVP cover film (UV compatible)
No cross contamination
Membrane protein crystallization
6 mm diameter drop well area
0.1 mm (100 μm) deep drop well when LCP plate is sealed
Maximum drop volume is 1.6 microliters per well

Description

The Lipidic Cubic Phase Crystallization (LCP) plate facilitates the automation and increased throughput of LCP crystallization set-ups. This novel system enables LCP screening to be performed accurately and with ease – using manual – or automated systems to complete the delivery of the solutions.

Working with innovative scientists Professor Gebhard Schertler and Pat Edwards from the MRC laboratory in Cambridge, Swissci AG developed a new system for setting up LCP screens.

The Swissci LCP plate ensures crystallography set-up is fast, cost effective and robotically adaptable. In addition Ultra Violet light can be used with the glass / UVP polymers to visualize proteins without the light scattering associated with traditional polymeric solutions.

The LCP technique for crystallizing membrane proteins can be difficult and time-consuming to set up as it utilizes highly viscous lipid mesophases to reconstitute proteins. Swissci AG and MRC overcame these problems with the LCP plate. This product combines an easy to use sandwich system with dedicated coupling tapes and thin film UVP cover plates. It conforms to SBS standards and thus is perfect for modern automation applications where high throughput is required.

The Advantages of the LCP plate
• Ready to use the plate fits laboratory robotics and uses a Swissci unique polymer to ensure UV visualization is not compromised by polarization.
• Easy sealing with dry tabbed adhesive tape exposure and thin UVP cover film. Included in the kit is a plate leveling device to ensure the cover is perfectly adhered to the bottom LCP plate.
• No cross contamination and SBS standard.
• Unique low tack plate security allows for the sandwich plate to be removed from the base plate when required – in-situ x-ray data collection and structure determination is then enabled.

Here’s a description of the LCP plate from bottom to top. a) An SBS standard base plate frame support. b) 0.7 mm thick UVP plastic slide drop support containing all 96 drop wells with 0.1 mm thick layer of low tack sealant (no sealant in drop area). Flat, circular, 6 mm diameter drop wells to contain the LCP, sample and reagent. c) Removable brown/tan paper protects sealant until plate is ready to use. d) 0.1 mm thick UVP sealing film. e) Removable protective dust cover. The kit is completed with a Swissci plate leveling device.

To use the LCP plate, pipette LCP, sample and reagent into the 96 drop wells. Peel back and remove the brown/tan paper to expose the adhesive. Place the sealing film onto the LCP plate. Remove the protective film for viewing.

Maximum drop volume is 1.6 microliters per well.

Click to Zoom In

CAT NO

HR3-186

NAME

Lipidic Cubic Phase Screening Kit

DESCRIPTION

20 plate case

PRICE

$422.00

Temporarily Unavailable
More Available Soon!

Support Material(s)

HR3-186 LCP Lipidic Cubic Phase Screen Kit User Guide

Hampton蛋白结晶试剂盒Diaplate

发表于2024年7月26日由上海金畔生物科技有限公司

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > 96 Well Crystallization Plates > Swissci > Diaplate