Hampton蛋白结晶试剂盒Sodium acetate trihydrate Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

应用

  • Crystallization grade Sodium acetate trihydrate buffer for formulating screens or for optimization
  • 结晶级三水乙酸钠缓冲液,用于配制筛选或优化

特征

  • Sterile filtered 无菌过滤
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Formulated from Sodium acetate trihydrate

由醋酸钠三水合物配制而成

Synonyms: Acetic acid sodium salt trihydrate

别名:醋酸钠盐三水合物
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%
pKa = 4.8

The useful pH range of a Sodium acetate trihydrate buffer is pH 3.6 – 5.6.

Titrate HR2-569 to the desired pH using HCl
HR2-569 Measured pH Range: 8.7 – 9.3 at 25°C
HR2-569 Measured Conductivity Range: 48.0 – 51.7 mS/cm at 25°C
HR2-569 Measured Refractive Index Range: 1.34395 – 1.34415 at 20°C

HR2-789 has been titrated to pH 4.5 using HCl
HR2-789 Measured Conductivity Range: 60.8 – 67.7 mS/cm at 25°C
HR2-789 Measured Refractive Index Range: 1.34530 – 1.34579 at 20°C

HR2-731 has been titrated to pH 4.6 using HCl
HR2-731 Measured Conductivity Range: 59.3 – 64.5 mS/cm at 25°C
HR2-731 Measured Refractive Index Range: 1.34535 – 1.345745 at 20°C

Al  ≤0.0005%
As ≤0.00001%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni  ≤0.0005%
Pb  ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr  ≤0.0005%
Zn  ≤0.0005%

Sodium acetate trihydrate

Sodium acetate trihydrate Buffer
CAT NO

HR2-569

NAME

1.0 M Sodium acetate trihydrate

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-789

NAME

1.0 M Sodium acetate trihydrate pH 4.5

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-731

NAME

1.0 M Sodium acetate trihydrate pH 4.6

DESCRIPTION

100 mL

PRICE

$41.00

相关项目

  • Hydrochloric acid 盐酸
  • StockOptions Sodium Acetate Buffer Kit StockOptions 醋酸钠缓冲液套件
  • Individual StockOptions Sodium Acetate Reagents 个人股票期权乙酸钠试剂

参考

1. Crystal structure of thioltransferase at 2.2 A resolution. Katti, SK; Robbins, AH; Yang, Y; Wells, WW. Protein Sci 4, 1998- 2005, 1995.

Hampton蛋白结晶试剂盒ADA Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > ADA Buffer

ADA Buffer

应用

  • Crystallization grade ADA buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 ADA 缓冲液

特征

  • Sterile filtered 无菌过滤
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

ADA (Buffer)

Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
Mr 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius

Useful pH range of ADA is 5.6 – 7.5

Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH

HR2-507 measured Conductivity range: 23.6 – 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 – 1.35044 at 20°C

HR2-817 measured Conductivity range: 42.2 – 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 – 1.36913 at 20°C

ADA buffer is used in the Hampton Research MembFac crystallization kit.ADA 缓冲液用于 Hampton Research MembFac 结晶试剂盒。

Total Impurities Insoluble matter, passes filter test总杂质 不溶物,通过过滤测试
Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.1%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

ADA Buffer
CAT NO

HR2-507

NAME

0.5 M ADA

DESCRIPTION

100 mL

PRICE

$123.00

CAT NO

HR2-817

NAME

1.0 M ADA pH 6.5

DESCRIPTION

100 mL

PRICE

$132.00

SDS

相关项目

  • Sodium hydroxide 氢氧化钠
  • Individual StockOptions ADA Reagents 个人股票期权 ADA 试剂
  • StockOptions ADA Buffer Kit StockOptions ADA 缓冲套件

参考

1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.

2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.

1.人和牛凝血酶原片段2与人PPACK-凝血酶的非共价复合物的结构。 阿尼,RK; 帕德马纳班,K; 帕德马纳班,KP; 吴,TP; Tulinsky, A. Biochemistry 32, 4727-4737, 1993。

2. 甜蛋白奇异果甜蛋白的三种晶型结构。 高,TP; 日,J; 格林伍德,一个; McPherson, A. 晶体学学报 D 50, 813-825, 1994。

Hampton蛋白结晶试剂盒Acetic acid

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Acetic acid

Acetic acid

应用

  • Crystallization grade Acetic acid for altering drop and reservoir pH
  • 结晶级乙酸,用于改变液滴和储液器的 pH 值

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Acetic acid

Synonyms: Glacial acetic acid
CAS Number [64-19-7]
CH3CO2H
Mr 60.05
Beilstein Registry Number 506007
EC Number 200-580-7
MDL number MFCD00036152
PubChem Substance ID 24859247

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

别名:冰醋酸
CAS 编号 [64-19-7]
CH3CO2H
60.05 先生
贝尔斯坦注册号 506007
欧盟编号 200-580-7
MDL 编号 MFCD00036152
PubChem 物质 ID 24859247

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法,请参阅 pdf 使用挥发性缓冲液。

Acetic acid

Acetic acid
CAT NO

HR2-853

NAME

5.2 M Acetic acid

DESCRIPTION

15 mL

PRICE

$24.00

相关项目

  • Ammonium hydroxide
  • 氢氧化铵

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

3. Crystallization and preliminary X-ray diffraction studies of the engrailed homeodomain and of an engrailed homeodomain/DNA complex. Liu et al, Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 1990, pages 257-259.

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

7. The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue. Muranova et al, Acta Cryst. (2001). D57, 1192±1195

1. 蛋白质晶体的制备和分析。 Alexander McPherson,1982,John Wiley and Sons, Inc.,由 Krieger Publishing 印刷。第 4 章,结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森,欧元。 J.生化。 189, 1-23 (1990)。

3. engrailed homeodomain 和 engrailed homeodomain/DNA 复合物的结晶和初步 X 射线衍射研究。刘等人,生化和生物物理研究通讯,卷。 171,第 1 期,1990 年,第 257-259 页。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人,J. Appl。水晶。 (1989 年)。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。 (1989 年)。 22、115-118

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人,Acta Cryst。 (2003 年)。 D59,1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人,Acta Cryst。 (2001 年)。 D57, 1192±1195

Hampton蛋白结晶试剂盒Ammonium hydroxide

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Ammonium hydroxide

Ammonium hydroxide

应用

  • Crystallization grade Ammonium hydroxide for altering drop and reservoir pH
  • 用于改变液滴和油藏 pH 值的结晶级氢氧化铵

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium hydroxide

Synonyms: Ammonia aqueous, Ammonia water
CAS Number [1336-21-6]
NH4OH
Mr 35.05
Beilstein Registry Number 3587154
EC Number 215-647-6
MDL Number MFCD00066650

See the pdf Using Volatile Buffers for a novel crystallization methodology using acetic acid and ammonium hydroxide to adjust drop pH and induce crystallization.

同义词:氨水、氨水
CAS 编号 [1336-21-6]
NH4OH
35.05 先生
贝尔斯坦注册号 3587154
欧盟编号 215-647-6
MDL 编号 MFCD00066650

有关使用乙酸和氢氧化铵调节液滴 pH 值并诱导结晶的新型结晶方法,请参阅 pdf 使用挥发性缓冲液。

Ammonium hydroxide

Ammonium hydroxide
CAT NO

HR2-855

NAME

5.2 M Ammonium hydroxide

DESCRIPTION

10 mL

PRICE

$24.00

 

相关项目

  • Acetic acid 醋酸

参考

1. Preparation and analysis of protein crystals. Alexander McPherson, 1982, John Wiley and Sons, Inc., printed by Krieger Publishing. Chapter 4, Crystallization.

2. Current approaches to macromolecular crystallization. Alexander McPherson, Eur. J. Biochem. 189, 1-23 (1990).

3. Crystallization and preliminary X-ray diffraction studies of the engrailed homeodomain and of an engrailed homeodomain/DNA complex. Liu et al, Biochemical and Biophysical Research Communications, Vol. 171, No. 1, 1990, pages 257-259.

4. Changes of pH during Biomacromolecule Crystallization by Vapor Diffusion using Ammonium Sulfate as the Precipitant. Mikol et al, J. Appl. Cryst. (1989). 22, 155-161.

5. A Double Cell for Controlling Nucleation and Growth of Protein Crystals. Maria Przybylska, J. Appl. Cryst. (1989). 22, 115-118.

6. Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid. Diao et al, Acta Cryst. (2003). D59, 1100±1102.

7. The preparation and crystallization of Fab fragments of a family of mouse esterolytic catalytic antibodies and their complexes with a transition-state analogue. Muranova et al, Acta Cryst. (2001). D57, 1192±1195.

1. 蛋白质晶体的制备和分析。 Alexander McPherson,1982,John Wiley and Sons, Inc.,由 Krieger Publishing 印刷。第 4 章,结晶。

2. 大分子结晶的当前方法。亚历山大麦克弗森,欧元。 J.生化。 189, 1-23 (1990)。

3. engrailed homeodomain 和 engrailed homeodomain/DNA 复合物的结晶和初步 X 射线衍射研究。刘等人,生化和生物物理研究通讯,卷。 171,第 1 期,1990 年,第 257-259 页。

4. 使用硫酸铵作为沉淀剂通过蒸汽扩散进行生物大分子结晶过程中 pH 值的变化。 Mikol 等人,J. Appl。水晶。 (1989 年)。 22, 155-161。

5. 用于控制蛋白质晶体成核和生长的双细胞。 Maria Przybylska, J. Appl.水晶。 (1989 年)。 22, 115-118。

6. 乙酸蒸汽扩散介导的来自海栖热袍菌的丁酸激酶 2 的结晶。刁等人,Acta Cryst。 (2003 年)。 D59,1100±1102。

7.小鼠酯水解催化抗体家族的Fab片段及其与过渡态类似物的复合物的制备和结晶。 Muranova 等人,Acta Cryst。 (2001 年)。 D57,1192±1195。

Hampton蛋白结晶试剂盒BICINE Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BICINE Buffer

BICINE Buffer

应用

  • Crystallization grade BICINE buffer for formulating screens or for optimization
  • 用于配制屏幕或优化的结晶级 BICINE 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BICINE

Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
Mr 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
PubChem Substance ID 24891738
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C

Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 – 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 – 167.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 – 1.35821 at 20°C

HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 – 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 – 1.36122 at 20°C

同义词: N,N-双(2-羟乙基)甘氨酸
C6H13NO4
163.17先生
CAS 编号 [150-25-4]
欧盟编号 205-755-1
贝尔斯坦注册号 1769362
默克 14,1201
RTECS MB9700000
MDL 编号 MFCD00004295
PubChem 物质 ID 24891738
纯度≥99.0%
pKa 在 20°C 时为 8.3,在 25°C 时为 8.3

有用的 pH 范围 7.4-9.3
使用 NaOH 将 HR2-509 滴定至有用的 pH 范围
HR2-509 pH 范围:25°C 时为 5.0 – 5.3
HR2-509 测量电导率范围:25°C 时为 100.7 – 167.2 µS/cm
HR2-509 测量的折射率范围:20°C 时为 1.35769 – 1.35821

HR2-723 已使用 NaOH 滴定至 pH 9.0
HR2-723 测得的电导率范围:23.9 – 25.0 mS/cm,25°C
HR2-723 测量的折射率范围:20°C 时为 1.36054 – 1.36122

BICINE

BICINE Buffer
CAT NO

HR2-509

NAME

1.0 M BICINE

DESCRIPTION

100 mL

PRICE

$104.00

CAT NO

HR2-723

NAME

1.0 M BICINE pH 9.0

DESCRIPTION

100 mL

PRICE

$104.00

相关项目

  • Sodium hydroxide
  • Individual StockOptions Bicine Reagents
  • StockOptions Bicine Buffer Kit
  • 氢氧化钠
    个别股票期权 Bicine 试剂
    StockOptions Bicine 缓冲套件

参考

1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.

1. 来自 Ophiostoma novo-ulmi 的真菌水解酶的结晶和初步 X 射线衍射研究。 M. N. Isupov、A. A. Brindley、E. J. Hollingsworth、G. N. Murshudov、A. A. Vagin 和 J. A. Littlechild。 晶体学报。 (2004 年)。 D60,1879-1882。

Hampton蛋白结晶试剂盒BIS-TRIS Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer

BIS-TRIS Buffer

应用

  • Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 BIS-TRIS 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS

Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C

HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C

HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C

同义词:2,2-双(羟甲基)-2,2′,2″”-次氮基三乙醇,或 2-双(2-羟乙基)氨基-2-(羟甲基)-1,3-丙二醇,或双(2- 羟乙基)氨基-三(羟甲基)甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C

使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围:25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率:20°C 时为 1.37069 – 1.37150

HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围:22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率:20°C 时为 1.36817 – 1.36926

Al  ≤0.0005%
Ca ≤0.0005%
Cl  ≤0.05%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na  ≤0.005%
Pb  ≤0.001%
SO4 ≤0.05%
Zn  ≤0.0005%

BIS-TRIS

BIS-TRIS Buffer
CAT NO

HR2-781

NAME

1.0 M BIS-TRIS pH 5.5

DESCRIPTION

100 mL

PRICE

$169.00

CAT NO

HR2-783

NAME

1.0 M BIS-TRIS pH 6.5

DESCRIPTION

100 mL

PRICE

$169.00

相关项目

  • Hydrochloric acid
  • StockOptions Bis-Tris Buffer Kit
  • Individual StockOptions Bis-Tris Reagents
  • 盐酸
    StockOptions Bis-Tris 缓冲套件
    个别股票期权 Bis-Tris 试剂

参考

1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.

2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.

1. 噬菌体 G4 降解前衣壳颗粒的原子结构:在钙离子存在下诱导结构变化和功能影响。 麦肯纳,R; 鲍曼,BR; 伊拉格,LL; 罗斯曼,MG; 法恩,文学士。 分子生物学杂志 256, 736-750, 1996。

2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。 蒙托亚,G; 斯文森,C; 卢林克,J; Sinning, I. 蛋白质 28, 285-288, 1997。

Hampton蛋白结晶试剂盒Parabar 10312 (previously known as Paratone)

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

应用

  • Oil based cryoprotectant

特征

  • Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
  • Oil based cryoprotectant

描述

Sorry, Parabar 10312 is no longer available.  We’ve left this page up for reference only.  See HR2-861 Santovac Cryo Oil as a possible substitute.

抱歉,Parabar 10312 不再可用。 我们保留此页面仅供参考。 请参阅 HR2-861 Santovac Cryo Oil 作为可能的替代品。

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512.

Paratone® is a registered trademark of Chevron Corporation.

Parabar 10312 是一种粘性冷冻保护剂,适用于小分子和大分子晶体学。 1

可以在惰性气氛下用 Parabar 包覆对空气轻微不稳定的小分子化合物。 Parabar 保护的晶体样品可以在冷冻氮气流中进行低温冷却。 Parabar 也已成功用作生物大分子晶体的冷冻保护剂。在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。用 Parabar 涂覆晶体可以最大限度地减少晶体的蒸发,并减少空气(氧气)向晶体的暴露和缓慢扩散。

HR2-643 Parabar 10312 不再可用,自 2021 年 5 月 11 日起停产。

HR2-862 Parabar 10312 以 5 ml 装在琥珀色玻璃小瓶中,螺旋盖盖中带有玻璃涂抹棒。

通过将 50:50 与石蜡油混合,可以降低 Parabar 的粘度,以改善易碎针状和刀片状晶体的处理。

Parabar 是聚异丁烯的混合物,Staudinger 分子量约为 20,000,含有约 20% 的聚合物。

20°C 时的折射率为 1.48487。

100°C 时运动粘度为 641 cSt。 100°C 时运动粘度比为 2.05。

清晰到略微朦胧的是外观。

晶体学文献中使用的同义词:Paratone® N、Paratone® 8277、润英联 V8512。

Paratone® 是雪佛龙公司的注册商标。

Parabar 10312

Parabar 10312 (previously known as Paratone)
Parabar 10312 (previously known as Paratone)

相关项目

  • Perfluoropolyether Cryo Oil
  • Santovac Cryo Oil
  • 全氟聚醚冷冻油
    Santovac 冷冻油

参考

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.

1. H. Hope,生物大分子的低温晶体学:一种普遍适用的方法。 晶体学报。 (1988) B44,22-26。

2. 与选择性调节剂 LGD2226 复合的人雄激素受体的配体结合域 (LBD) 的结构。 F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi 和 T. Jiang。 晶体学报。 (2006 年)。 F62, 1067-1071。

3. H.霍普,安努。 牧师生物物理学。 化学。 1990 年 19:107-126。

4. S. Parkin 和 H. Hope,J. Appl。 水晶。 (1998) 第 945-953 页。

Hampton蛋白结晶试剂盒BIS-TRIS propane Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS propane Buffer

BIS-TRIS propane Buffer

应用

  • Crystallization grade BIS-TRIS propane pH 7.0 for formulating screens or for optimization
  • 结晶级 BIS-TRIS 丙烷 pH 7.0,用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS propane

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
Mr 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C

HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C

别名:1,3-双[三(羟甲基)甲氨基]丙烷
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
282.34 先生
CAS 编号 [64431-96-5]
欧盟编号 264-899-3
贝尔斯坦注册号 1786109
MDL 编号 MFCD00004689
PubChem 物质 ID 24891934
纯度≥99.0%
pKa1 = 6.8 在 25°C
pKa2 = 9.0 在 25°C

HR2-795 使用 HCl 滴定至 pH 7.0
测得的电导率范围:25°C 时 41.9 – 43.5 mS/cm
测得的折射率范围:20°C 时为 1.39110 – 1.39222

BIS-TRIS propane

BIS-TRIS propane Buffer
CAT NO

HR2-795

NAME

1.0 M BIS-TRIS propane pH 7.0

DESCRIPTION

100 mL

PRICE

$108.00

相关项目

  • Individual StockOptions Bis-Tris Propane Reagents
  • Citric acid BIS-TRIS propane Buffer
  • 个别股票期权 Bis-Tris 丙烷试剂
    柠檬酸 BIS-TRIS 丙烷缓冲液

参考

1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.

2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.

1. 结晶 DNA-EcoRI 核酸内切酶识别复合物的双重对称性。 格拉布尔,J; 加利福尼亚州弗雷德里克; 萨穆兹,C; 仁-雅各布森,L; 较小,D; 格林,P; 博耶,HW; 板仓,K; 罗森伯格,JM。 J Biomol Struct Dyn 1, 1149-1160, 1984。

2. 蛋白质折叠和稳定性中的非天然局部相互作用:在 AII β-片 α-Spectrin SH3 域中引入螺旋趋势。 普列托,J; 威尔曼斯,M; 马萨诸塞州希门尼斯; 里科,M; Serrano, L. J Mol Biol 268, 760-778, 1997。

Hampton蛋白结晶试剂盒Citric acid Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Citric acid Buffer

Citric acid Buffer

应用

  • Crystallization grade Citric acid buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级柠檬酸缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Citric acid

Synonyms: Citric acid anhydrous
C6H8O7
HOC(COOH)(CH2COOH)2
Mr 192.13
CAS Number [77-92-9]
EC Number 201-069-1
Beilstein Registry Number 782061
Merck 14,2326
RTECS GE7350000
MDL Number MFCD00011669
PubChem Substance ID 24856611
Purity ≥ 99.5%
Useful pH range of citric acid as a buffer 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 is titrated to pH 3.5 at 25 degrees Celsius using NaOH
Measured Conductivity Range: 28.7 – 33.3 mS/cm at 25°C
Measured Refractive Index Range: 1.36236 – 1.36329 at 20°C

别名: 无水柠檬酸
C6H8O7
HOC(COOH)(CH2COOH)2
192.13 先生
CAS 编号 [77-92-9]
欧盟编号 201-069-1
贝尔斯坦注册号 782061
默克 14,2326
RTECS GE7350000
MDL 编号 MFCD00011669
PubChem 物质 ID 24856611
纯度≥99.5%
柠檬酸作为缓冲液的有用 pH 值范围 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 在 25 摄氏度下使用 NaOH 滴定至 pH 3.5
测得的电导率范围:25°C 时 28.7 – 33.3 mS/cm
测得的折射率范围:20°C 时为 1.36236 – 1.36329

Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Oxalate (C2O4) ≤0.05%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr ≤0.0005%
Tartrate (C4H4O6) ≤0.2%
Zn ≤0.0005%

Citric acid

Citric acid Buffer
CAT NO

HR2-757

NAME

1.0 M Citric acid pH 3.5

DESCRIPTION

100 mL

PRICE

$44.00

相关项目

  • Sodium hydroxide
  • StockOptions Citric Acid Buffer Kit
  • Individual StockOptions Citric Acid Reagents
  • 氢氧化钠
    StockOptions 柠檬酸缓冲液套件
    个别股票期权柠檬酸试剂

参考

1. Crystallization and preliminary X-ray structure solution of Rhizomucor miehei aspartic proteinase. Jia, Z; Vandonselaar, M; Schneider, P; Quail, JW. Acta Crystallogr D 51, 243- 244, 1995.

1.米黑根毛霉天冬氨酸蛋白酶的结晶和初步X射线结构解。 贾,Z; 范登塞拉,M; 施耐德,P; 鹌鹑,JW。 晶体学学报 D 51, 243-244, 1995。

Hampton蛋白结晶试剂盒Citric acid BIS-TRIS propane Buffer

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Citric acid BIS-TRIS propane Buffer

Citric acid BIS-TRIS propane Buffer

应用

  • Crystallization grade CBTP buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 CBTP 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Citrate BIS-TRIS propane (CBTP) is a buffer system useful across pH 2.5 to 9.5.

Using CBTP one can use a single buffer system to screen pH 2.5 to 9.5 by varying the ratio of Citric acid to BIS-TRIS propane. Although the two buffer reagents are made available separately for convenient, individual replacement, they are designed to be used together, with the supplied CBTP buffer titration table to create a crystallization buffer system covering pH 2.5 to 9.5.

HR2-831 Measured pH range: 1.2 – 1.5 at 25°C
HR2-831 Measured Conductivity Range: 7.7 – 8.7 mS/cm at 25°C

HR2-833 Measured pH range: 11.2 – 11.5 at 25°C
HR2-833 Measured Conductivity Range: 298 – 317 µS/cm at 25°C
HR2-833 Measured Refractive Index Range: 1.38000 – 1.38076 at 20°C

柠檬酸盐 BIS-TRIS 丙烷 (CBTP) 是一种适用于 pH 2.5 至 9.5 的缓冲系统。

使用 CBTP,可以使用单一缓冲系统通过改变柠檬酸与 BIS-TRIS 丙烷的比例来筛选 pH 2.5 至 9.5。 虽然这两种缓冲试剂是分开提供的,以便于单独更换,但它们被设计为一起使用,与提供的 CBTP 缓冲液滴定表一起创建一个涵盖 pH 2.5 至 9.5 的结晶缓冲系统。

HR2-831 测得的 pH 值范围:25°C 时为 1.2 – 1.5
HR2-831 测量电导率范围:25°C 时 7.7 – 8.7 mS/cm

HR2-833 测得的 pH 范围:25°C 时为 11.2 – 11.5
HR2-833 测量的电导率范围:298 – 317 µS/cm,25°C
HR2-833 测量的折射率范围:20°C 时为 1.38000 – 1.38076

Citric acid

Citric acid BIS-TRIS propane Buffer
Citric acid BIS-TRIS propane Buffer
CAT NO

HR2-831

NAME

1.0 M Citric acid

DESCRIPTION

100 mL

PRICE

$45.00

CAT NO

HR2-833

NAME

1.0 M BIS-TRIS propane

DESCRIPTION

100 mL

PRICE

$162.00