HR4-672

发表于2024年7月25日由上海金畔生物科技有限公司

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Products > Cryocrystallography > Cryo Tools > Vial Clamp™ – Curved

Vial Clamp™ – Curved

应用

Vial support & manipulation
样品瓶支持和操作

特征

Curved end tip
Hemostat style closure
弯曲端头
止血式封口

描述

The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.

Vial Clamp – Curved 是一种镀铬的止血工具。它有一个尖端，可在夹子闭合时以一定角度固定所有 CrystalCap 系统的存储瓶。可以使用止血器式闭合来锁定夹子。夹持小瓶的夹具末端以 45°/135° 或 110°/70° 角弯曲。当小瓶放置在夹具中时，它的长度以这些相同角度中的任何一个定位到夹具。每个夹具的总长度相同，均为 195 毫米。

HR4-671 Vial Clamp Curved 45°/135°

CAT NO

HR4-671

NAME

Vial Clamp – Curved 45°/135°

DESCRIPTION

each

PRICE

$67.00

CAT NO

HR4-672

NAME

Vial Clamp – Curved 110°/70°

DESCRIPTION

each

PRICE

$97.00

Hampton蛋白结晶试剂盒Santovac Cryo Oil/HR2-861

发表于2024年6月18日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Cryoprotectants > Santovac Cryo Oil

Santovac Cryo Oil

CAT NO

HR2-861

NAME

Santovac Cryo Oil

DESCRIPTION

1 mL

PRICE

$26.00

应用

Oil based cryoprotectant
油基防冻剂

特征

Low viscosity
Low surface tension
Cryoprotectant for soluble and membrane protein crystals
Not miscible with most solvents
Non toxic, non penetrating cryoprotectant
低粘度
低表面张力
可溶性和膜蛋白晶体的冷冻保护剂
不与大多数溶剂混溶
无毒、非渗透性冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Santovac Cryo Oil offers low viscosity, incredibly low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Santovac to the crystallization drop before mounting the crystal. After adding the Santovac, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Santovac will coat the mounted crystal. Cryogenically cool the mounted crystal.

Santovac (Santovac 5) is a five ring polyphenyl ether (PPE) with very low vapor pressure and low reactivity. The oil is highly resistant to oxidation. The only elements in the pure polyphenyl ether are oxygen, hydrogen and carbon.

The fluid is chemically stable, non-corrosive, safe and non-toxic at normal operating temperatures.

Santovac is not miscible with water, paraffin oil, silicon oil, alkanes, or Parabar 10312 (Paratone). Santovac is miscible with acetone. Shelf life 5 years.

Santovac^® is a trademark of Santovac^® Fluids.

在晶体安装在低温回路中后，在低温晶体学期间用于置换和减少晶体上的水（母液、试剂）量。用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气（氧气）向晶体的暴露和缓慢扩散。

Santovac Cryo Oil 具有低粘度、极低的蒸气压、低表面张力、出色的化学惰性和出色的热稳定性。

将安装的晶体浸入油中以置换表面水。或者在安装晶体之前将少量的 Santovac 添加到结晶液滴中。添加 Santovac 后，使用 Mounted Cryoloop 安装晶体。从水滴中取出已镶嵌的水晶，Santovac 将覆盖已镶嵌的水晶。低温冷却安装的晶体。

Santovac (Santovac 5) 是一种五环聚苯醚 (PPE)，具有极低的蒸气压和低反应性。该油具有很强的抗氧化性。纯聚苯醚中唯一的元素是氧、氢和碳。

该流体在正常工作温度下化学性质稳定、无腐蚀性、安全无毒。

Santovac 与水、石蜡油、硅油、烷烃或 Parabar 10312 (Paratone) 不混溶。 Santovac 可与丙酮混溶。保质期5年。

Santovac® 是 Santovac® Fluids 的商标。

Santovac Cryo Oil

支持材料

HR2-861 Santovac Cryo Oil User Guide HR2-861 Santovac Cryo Oil SDS

参考

1. Structure of the ‘open’ form of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution from crystal grown following enzyme turnover. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. Crystal Structure of the Caspase Activator Human Granzyme B, a Proteinase Highly Specific for an Asp-P1 Residue. E. Estebanez-Perpina et al. Biol. Chem., Vol. 381, pp. 1203-1214, December 2000.

1. 构巢曲霉 3-脱氢喹酸合酶“开放”形式的结构，分辨率为 1.7 Å，来自酶转换后生长的晶体。 C.E.尼科尔斯，A.R. 霍金斯和 D.K. 斯塔默萨。晶体学报 D 部分，第 60 卷，第 5 部分，第 971-973 页，2004 年 5 月。

2. Caspase 激活剂人粒酶 B 的晶体结构，一种对 Asp-P1 残基具有高度特异性的蛋白酶。 E. Estebanez-Perpina 等人。生物学。化学，卷。 381，第 1203-1214 页，2000 年 12 月。

Hampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735

发表于2024年6月18日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Buffers > Sodium citrate tribasic dihydrate Buffer

Sodium citrate tribasic dihydrate Buffer

CAT NO

HR2-571

NAME

1.0 M Sodium citrate tribasic dihydrate

DESCRIPTION

100 mL

PRICE

$56.00

CAT NO

HR2-735

NAME

1.0 M Sodium citrate tribasic dihydrate pH 5.6

DESCRIPTION

100 mL

PRICE

$56.00

支持材料

HR2-571 Sodium citrate tribasic dihydrate SDS

HR2-735 Sodium citrate tribasic dihydrate pH 5.6 SDS

Titration Table

应用

Crystallization grade Sodium citrate tribasic dihydrate buffer for formulating screens or for optimization
结晶级柠檬酸钠三水合物缓冲液，用于配制筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Sodium citrate tribasic dihydrate

Synonyms: Citric acid trisodium salt dihydrate or Trisodium citrate dihydrate
C₆H₅Na₃O₇• 2H₂O
HOC(COONa)(CH₂COONa)₂• 2H₂O
M_r 294.10
CAS Number [6132-04-3]
EC Number 200-675-3
Merck 14,8602
Beilstein Registry Number 6104939
MDL Number MFCD00150031
Purity ≥ 99.0%

Useful pH range of a Sodium citrate tribasic dihydrate buffer: 2.2 – 6.5

Titrate HR2-571 to the desired pH using HCl
HR2-571 Measured pH Range: 8.1 – 9.4 at 25°C
HR2-571 Measured Conductivity Range: 60.0 – 69.8 mS/cm at 25°C
HR2-571 Measured Refractive Index Range: 1.37345 – 1.37370 at 20°C

HR2-735 is titrated to pH 5.6 using HCl
HR2-735 Measured Conductivity Range: 67.4 – 78.5 mS/cm at 25°C
HR2-735 Measured Refractive Index Range: 1.37400 – 1.37502 at 20°C

别名：柠檬酸三钠盐二水合物或柠檬酸三钠二水合物
C6H5Na3O7 • 2H2O
HOC(COONa)(CH2COONa)2 • 2H2O
294.10 先生
CAS 编号 [6132-04-3]
欧盟编号 200-675-3
默克 14,8602
贝尔斯坦注册号 6104939
MDL 编号 MFCD00150031
纯度≥99.0%

柠檬酸钠三水合物缓冲液的有用 pH 范围：2.2 – 6.5

使用 HCl 将 HR2-571 滴定至所需的 pH 值
HR2-571 测量的 pH 范围：25°C 时 8.1 – 9.4
HR2-571 测量的电导率范围：25°C 时为 60.0 – 69.8 mS/cm
HR2-571 测量的折射率范围：20°C 时为 1.37345 – 1.37370

使用 HCl 将 HR2-735 滴定至 pH 5.6
HR2-735 测得的电导率范围：25°C 时为 67.4 – 78.5 mS/cm
HR2-735 测量的折射率范围：20°C 时为 1.37400 – 1.37502

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.005%
Cd ≤0.0005%
Cl ≤0.001%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.01%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
NH₄⁺ ≤0.001%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Zn ≤0.0005%

Sodium citrate tribasic dihydrate

参考

1. Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1. Greasley, S; Jhoti, H; Fensome, AC; Cockcroft, S; Thomas, GM; Bax, B. J Mol Biol 244, 651- 653, 1994.

1. ADP-核糖基化因子的结晶和初步 X 射线衍射研究 1. Greasley, S; 乔蒂，H；芬索姆，AC；考克罗夫特，S；托马斯，总经理； Bax, B. J Mol Biol 244, 651-653, 1994。

Hampton蛋白结晶试剂盒TMAO/HR2-857

发表于2024年6月17日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Cryoprotectants > TMAO

TMAO

CAT NO

HR2-857

NAME

4.0 M TMAO

DESCRIPTION

1 mL

PRICE

$26.00

支持材料

HR2-857 4.0 M TMAO SDS

应用

Water soluble cryoprotectant
水溶性冷冻保护剂

特征

A very versatile cryoprotectant for macromolecular crystals
Protein stabilizer
TMAO can be a viable alternative when conventional cryoprotectants fail
Low viscosity and low surface tension
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
一种非常通用的大分子晶体冷冻保护剂
蛋白质稳定剂
当传统冷冻保护剂失效时，TMAO 可能是一种可行的替代方案
低粘度和低表面张力
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Trimethylamine N-oxide dihydrate (TMAO)

Formulated from TMAO dihydrate in Type 1 lab water (deionized) and sterile filtered (0.22 micron)

Synonyms: TMANO, TMAO
(CH₃)₃NO•2H₂O
C₃H₉NO•2H₂O
M_r 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
Purity ≥ 98.0%
Measured pH range: 9.2 – 10.6 at 25°C
Measured Conductivity Range: 5.6 – 96.6 µS/cm at 25°C
Measured Refractive Index Range: 1.37660 – 1.37804 at 20°C

When using TMAO as a cryoprotectant, try soaking the crystals in 4.0 M TMAO for 10 seconds and then cryo cool directly in the cryo stream.

由 1 型实验室用水（去离子）和无菌过滤（0.22 微米）中的 TMAO 二水合物配制而成

同义词：TMANO、TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
纯度≥98.0%
测得的 pH 值范围：25°C 时 9.2 – 10.6
测得的电导率范围：25°C 时 5.6 – 96.6 µS/cm
测得的折射率范围：20°C 时为 1.37660 – 1.37804

当使用 TMAO 作为冷冻保护剂时，尝试将晶体浸泡在 4.0 M TMAO 中 10 秒，然后直接在冷冻流中冷冻冷却。

参考

1. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Christoph Mueller-Dieckmann, Brice Kauffmann and Manfred S. Weiss. J. Appl. Cryst. (2011). 44, 433–436 doi:10.1107/S0021889811000045.

1. 三甲胺 N-氧化物作为大分子晶体学中的多功能冷冻保护剂。 Christoph Mueller-Dieckmann、Brice Kauffmann 和 Manfred S. Weiss。 J.应用。水晶。（2011）。 44, 433–436 doi:10.1107/S0021889811000045。

Hampton蛋白结晶试剂盒NDSB-195/HR2-703

发表于2024年6月16日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195

NDSB-195

CAT NO

HR2-703

NAME

NDSB-195

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

HR2-703 NDSB-195 User Guide

HR2-703 NDSB-195 SDS

应用

Crystallization grade NDSB-195 for formulating screens or for optimization
结晶级 NDSB-195 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C₇H₁₇NO₃S
M_r 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。

蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。

NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存

HR2-703

参考

1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.

2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.

4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

发表于2024年6月15日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

HR2-701 NDSB-201 User Guide

HR2-701 NDSB-201 SDS

应用

Crystallization grade NDSB-201 for formulating screens or for optimization
结晶级 NDSB-201 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C₈H₁₁NO₃S
M_r 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581

发表于2024年6月14日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Buffers > Hydrochloric acid

Hydrochloric acid

CAT NO

HR2-581

NAME

1.0 M Hydrochloric acid

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

HR2-581 Hydrochloric acid SDS

应用

Crystallization grade Hydrochloric acid for formulating screens or for optimization
结晶级盐酸，用于筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Hydrochloric acid

Synonyms: None
HCl
M_r 36.46
CAS Number [7647-01-0]
EC Number 231-595-7
Beilstein Registry Number 1098214
Merck 14,4794
RTECS MW4025000
MDL Number MFCD00011324
PubChem Substance ID 329754537
UN Number (RIDADR) UN 1789 8/PG 2

Measured Conductivity Range: 320.4 – 378.2 mS/cm at 25°C

The 1.0 M Hydrochloric acid is formulated using pyrogen free (<0.03 Endotoxin (EU/ml)) water. The final 1.0 M Hydrochloric acid is not certified pyrogen free.

同义词：无
盐酸
36.46 先生
CAS 编号 [7647-01-0]
欧盟编号 231-595-7
贝尔斯坦注册号 1098214
默克 14,4794
RTECS MW4025000
MDL 编号 MFCD00011324
PubChem 物质 ID 329754537
联合国编号 (RIDADR) UN 1789 8/PG 2

测得的电导率范围：25°C 时 320.4 – 378.2 mS/cm

使用无热原 (<0.03 内毒素 (EU/ml)) 的水配制 1.0 M 盐酸。最后的 1.0 M 盐酸未经过认证无热原。

Ag ≤0.000001%
Al ≤0.000005%
As ≤0.0000005%
Ba ≤0.000002%
Bi ≤0.00001%
Br ≤0.005%
Ca ≤0.00005%
Cd ≤0.000001%
Co ≤0.000001%
Cr ≤0.000002%
Cu ≤0.000002%
Fe ≤0.00002%
Hg ≤0.00001%
K ≤0.00001%
Li ≤0.000001%
Mg ≤0.00001%
Mn ≤0.000001%
Mo ≤0.000002%
Na ≤0.00005%
NH₄⁺ ≤0.0001%
Ni ≤0.000002%
PO₄ ≤0.0005%
Pb ≤0.000002%
SO₄ ≤0.0001%
SO₃ ≤0.0001%
Sr ≤0.000001%
TI ≤0.000005%
Zn ≤0.000005%

Hydrochloric acid相关项目

BIS-TRIS Buffer
HEPES sodium Buffer
Imidazole Buffer
Sodium acetate trihydrate Buffer
Sodium cacodylate trihydrate Buffer
Sodium citrate tribasic dihydrate Buffer
Tris Buffer
BIS-TRIS 缓冲器
HEPES钠缓冲液
咪唑缓冲液
三水乙酸钠缓冲液
二甲胂酸钠三水合物缓冲液
柠檬酸钠二水合物缓冲液
Tris 缓冲区

Hampton蛋白结晶试剂盒Sodium hydroxide/HR2-583

发表于2024年6月13日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Buffers > Sodium hydroxide

Sodium hydroxide

CAT NO

HR2-583

NAME

1.0 M Sodium hydroxide

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

HR2-583 Sodium hydroxide SDS

应用

Crystallization grade Sodium hydroxide for formulating screens or for optimization
结晶级氢氧化钠，用于筛选或优化

特征

Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
无菌过滤
在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Sodium hydroxide

Synonym: Caustic soda
NaOH
HNaO
M_r 40.00
CAS [1310-73-2]
EC Number 215-185-5
Merck 14,8627
MDL Number MFCD00003548
PubChem Substance ID 24899773
UN Number UN 1823 8/PG 2
RTECS WB4900000
Purity ≥ 98.0%

Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.02%
Mg ≤0.0005%
P ≤0.0005%
Pb ≤0.001%
SO₄ ≤0.05%
Zn ≤0.0005%

Sodium hydroxide

Hampton蛋白结晶试剂盒NDSB-221/HR2-791

发表于2024年6月13日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-221

NDSB-221

CAT NO

HR2-791

NAME

NDSB-221

DESCRIPTION

5 grams

PRICE

$58.00

支持材料

HR2-791 NDSB-221 User Guide

HR2-791 NDSB-221 SDS

应用

Crystallization grade NDSB-221 for formulating screens or for optimization
结晶级 NDSB-221 用于配制筛选或优化

特征

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability
在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-221
Synonyms: Non-detergent sulfobetaine 221 or 3-(1-Methylpiperidinium)-1-propane Sulfonate
C₉H₁₉NO₃S
M_r 221.34
Purity: ≥99.0%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-791参考

1. Goldberg, M.E., et al. 1996. Folding & Design 1, 21.

2. Protein crystallography with non detergent sulfobetaines. L. Vuillard et al. J Cryst Growth (1996) 168 150-15.

3. Vuillard L, Rabilloud T, Goldberg ME, Eur J Biochem 1998 256(1):128-135. (protein folding).

Hampton蛋白结晶试剂盒Polyethylene glycol 200/HR2-601

发表于2024年6月12日由上海金畔生物科技有限公司

Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 200

Polyethylene glycol 200

CAT NO

HR2-601

NAME

100% Polyethylene glycol 200

DESCRIPTION

200 mL

PRICE

$42.00

支持材料

HR2-601 Polyethylene glycol 200 SDS

应用

Crystallization grade Polyethylene glycol 200 for formulating screens or for optimization
结晶级聚乙二醇 200，用于配制筛网或优化

描述Polyethylene glycol 200

Synonyms: PEG 200
H(OCH₂CH₂)_nOH
Average Formula weight (M_r) 200 (190-210)
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3600000
MDL Number MFCD00081839
PubChem Substance ID 24898381
Melting Point -65°C (lit.)
Density 1.1239 g/mL at 20°C
Vapor pressure <0.01 mm Hg at 20°C
Viscosity 4.3 cSt at 20°C
Refractive index N20/D 1.459 (lit.)
Aldehyde: None detected
Peroxide: None detected

Measured Conductivity Range: 2.5 – 9.7 µS/cm at 25°C
Measured Refractive Index Range: 1.46008 – 1.46124 at 20°C

同义词：PEG 200
H(OCH2CH2)nOH
平均配方重量 (Mr) 200 (190-210)
CAS 编号 [25322-68-3]
欧盟编号 500-038-2
默克 14,7568
RTECS TQ3600000
MDL 编号 MFCD00081839
PubChem 物质 ID 24898381
熔点 -65°C (lit.)
密度 1.1239 g/mL 在 20°C
蒸气压 <0.01 mm Hg at 20°C
粘度 4.3 cSt 在 20°C
折射率 N20/D 1.459 (lit.)
醛：未检测到
过氧化物：未检测到

测得的电导率范围：25°C 时为 2.5 – 9.7 µS/cm
测得的折射率范围：20°C 时为 1.46008 – 1.46124

Polyethylene glycol 200

参考

1. Crystallization and Preliminary Structural Studies of a Chorismate Mutase Catalytic Antibody Complexed With a Transition State Analog. Haynes, MR; Stura, EA; Hilvert, D; Wilson, IA. Proteins 18, 198- 200, 1994.

1. 与过渡态类似物复合的分支酸变位酶催化抗体的结晶和初步结构研究。海恩斯先生；斯图拉，EA；希尔弗特，D；威尔逊，IA。蛋白质 18、198-200、1994。