Hampton蛋白结晶试剂盒Sodium potassium phosphate (Quik Optimize)/HR2-223

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Products > Optimize Reagents > Optimize – Salts > Sodium potassium phosphate (Quik Optimize)

Sodium potassium phosphate (Quik Optimize)

Sodium potassium phosphate (Quik Optimize)
Sodium potassium phosphate (Quik Optimize)
Sodium potassium phosphate (Quik Optimize)
Sodium potassium phosphate (Quik Optimize)
CAT NO

HR2-223

NAME

Quik Optimize

DESCRIPTION

100 mL each

PRICE

$240.00

支持材料

Sodium potassium phosphate (Quik Optimize) HR2-223 Quik Optimize DocumentsSodium potassium phosphate (Quik Optimize) HR2-223 Quik Optimize SDSSodium potassium phosphate (Quik Optimize) HR2-223 Quik Optimize Dilution Table

相关项目

  • Potassium phosphate dibasic
  • Sodium phosphate monobasic monohydrate
  • Quik Screen
  • 磷酸氢二钾
    磷酸二氢钠一水合物
    快屏

应用

  • Optimization of Sodium potassium phosphate concentration and pH
  • 优化磷酸钠钾浓度和pH

特征

  • Sterile filtered solution
  • Supplied formulation guide allows for quick and simple pH and dilution grids
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    提供的配方指南允许快速和简单的 pH 值和稀释网格
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

The Quik Optimize reagent set is designed to deliver a flexible phosphate reagent based solubility and crystallization screening method for the crystallization of biological macromolecules. Quick Optimize allows convenient and simple reproduction of original Quik Screen conditions. The kit is especially useful for refinement and optimization of preliminary successful crystallization conditions determined using Quik Screen.

Quik Optimize is composed of two high purity, sterile filtered stock solutions, 4.0 M Sodium phosphate monobasic monohydrate (NaH2PO4 Mr 137.99 CAS Number [10049-21-5]) and 4.0 M Potassium phosphate dibasic (K2HPO4 Mr 174.18 CAS Number [7758-11-4]) formulated using ultra pure water. Final reagent pH is determined by the ratio of Sodium and Potassium phosphate reagents. The supplied dilution table makes concentration and pH determinations quick and simple. Reagent concentrations between 0.2 and 4.0 M in 0.2 M increments and pH values between 5.0 and 8.2 in 0.2 pH increments can be conveniently formulated with Quik Optimize.

The Quik Optimize formulation has been used successfully in both crystallographic and bioseparation applications for the crystallization of biological macromolecules 1-7. The phosphate reagent system in Quik Optimize is a stable, flexible, easy to reproduce, cost-effective, and simple way to screen and optimize crystallization conditions for biological macromolecules. Quik Optimize was developed by Macrocrystal Oy and is manufactured and distributed exclusively by Hampton Research.

Each Quik Optimize kit contains 4.0 M Sodium phosphate monobasic monohydrate and 4.0 M Potassium phosphate dibasic reagents, 100 ml each. These ready to use reagents are formulated using high purity salts with ultra pure water and are sterile filtered. Each kit contains a dilution table to create any pH (between 5.0 & 8.2) and any concentration (between 0.2 & 4.0 M) in 0.2 increments. Crystallization accessories are sold separately.

Quik Optimize 试剂套装旨在提供灵活的基于磷酸盐试剂的溶解度和结晶筛选方法，用于生物大分子的结晶。快速优化可以方便、简单地再现原始 Quik 屏幕条件。该试剂盒特别适用于改进和优化使用 Quik Screen 确定的初步成功结晶条件。

Quik Optimize 由两种高纯度、无菌过滤储备溶液组成：4.0 M 磷酸二氢钠一水合物（NaH2PO4 Mr 137.99 CAS 编号 [10049-21-5]）和 4.0 M 磷酸氢二钾（K2HPO4 Mr 174.18 CAS 编号 [7758-11] -4]) 使用超纯水配制。最终试剂的 pH 值由磷酸钠和磷酸钾试剂的比例决定。提供的稀释表使浓度和 pH 值的测定快速而简单。使用 Quik Optimize 可以方便地配制 0.2 和 4.0 M 之间的 0.2 M 增量的试剂浓度和 5.0 和 8.2 之间的 0.2 pH 增量的 pH 值。

Quik Optimize 配方已成功用于生物大分子 1-7 结晶的晶体学和生物分离应用。 Quik Optimize 中的磷酸盐试剂系统是一种用于筛选和优化生物大分子结晶条件的稳定、灵活、易于复制、经济高效且简单的方法。 Quik Optimize 由 Macrocrystal Oy 开发，由 Hampton Research 独家制造和分销。

每个 Quik Optimize 试剂盒包含 4.0 M 磷酸二氢钠一水合物和 4.0 M 磷酸氢二钾试剂，各 100 ml。这些即用型试剂使用高纯度盐和超纯水配制而成，并经过无菌过滤。每个试剂盒都包含一个稀释表，以 0.2 为增量创建任何 pH 值（5.0 和 8.2 之间）和任何浓度（0.2 和 4.0 M 之间）。结晶配件单独出售。

参考Sodium phosphate monobasic monohydrate

1. Matthews, B.W., et al., J. Mol. Bio. (1979) 130, 507-510.

2. Blow, D.M., Acta Cryst. (1958) 11, 125-126.

3. Perutz, M.F., et al., J. Mol. Bio. (1968) 33, 283-297.

4. Kretsinger, R.H., J. Mol. Bio. (1968) 38, 141-143.

5. Johnson, J.E., et al., J. Ultrastruc. Res. (1977) 61, 240-242.

6. Davies, D.R., et al., Proc. Natl. Acad. Sci. USA (1972) 69, 3689-3992

7. Stout, C.D., J. Biol. Chem. (1979) 254, 3598-3599.