Hampton蛋白结晶试剂盒Perfluoropolyether Cryo Oil/HR2-814

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Products > Optimize Reagents > Optimize – Cryoprotectants > Perfluoropolyether Cryo Oil

Perfluoropolyether Cryo Oil全氟聚醚冷冻油

Perfluoropolyether Cryo Oil

CAT NO

HR2-814

NAME

Perfluoropolyether Cryo Oil

DESCRIPTION

1 mL

PRICE

$33.00

应用

  • Oil based cryoprotectant
  • 油基防冻剂

特征

  • Perfluoropolyether cryo oil
  • Low viscosity
  • Low surface tension
  • Cryoprotectant for soluble and membrane protein crystals
  • 全氟聚醚冷冻油
    低粘度
    低表面张力
    可溶性和膜蛋白晶体的冷冻保护剂

描述

Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with oil can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

Perfluoropolyether Cryo Oil offers low viscosity, extremely low vapor pressure, low surface tension, excellent chemical inertness and excellent thermal stability.

Dip the mounted crystal into the oil to displace surface water. Or add a small amount of Perfluoropolyether to the crystallization drop before mounting the crystal. After adding the Perfluoropolyether, mount the crystal using a Mounted Cryoloop. Withdraw the mounted crystal from the drop and the Perfluoropolyether will coat the mounted crystal. Cryogenically cool the mounted crystal.

在晶体安装在低温回路中后,在低温晶体学期间用于置换和减少晶体上的水(母液、试剂)量。 用油涂抹晶体可以最大限度地减少晶体的蒸发并减少空气(氧气)向晶体的暴露和缓慢扩散。

全氟聚醚冷冻油具有低粘度、极低蒸气压、低表面张力、优异的化学惰性和优异的热稳定性。

将安装的晶体浸入油中以置换表面水。 或者在安装晶体之前在结晶液滴中加入少量的全氟聚醚。 添加全氟聚醚后,使用 Mounted Cryoloop 安装晶体。 从液滴中取出已镶嵌的晶体,全氟聚醚将覆盖已镶嵌的晶体。 低温冷却安装的晶体。

相关项目

  • CryoPro

参考

1. Structure of the ‘open form’ of Aspergillus nidulans 3-dehydroquinate synthase at 1.7 Å resolution. C.E. Nichols, A.R. Hawkins and D.K. Stammersa. Acta Crystallographic Section D, Volume 60, Part 5, Pages 971-973, May 2004.

2. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.
1. 构巢曲霉 3-脱氢喹酸合酶“开放形式”的结构,分辨率为 1.7 Å。 C.E.尼科尔斯,A.R. 霍金斯和 D.K. 斯塔默萨。 晶体学报 D 部分,第 60 卷,第 5 部分,第 971-973 页,2004 年 5 月。

2. H.希望,安努。 牧师生物物理学。 化学。 1990 年 19:107-126。