Hampton蛋白结晶试剂盒HEPES sodium Buffer

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Products > Optimize Reagents > Optimize – Buffers > HEPES sodium Buffer

HEPES sodium Buffer

应用

  • Crystallization grade HEPES sodium buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 HEPES 钠缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述HEPES sodium

Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2

别名:HEPES钠盐,或4-(2-羟乙基)哌嗪-1-乙磺酸钠盐,或N-(2-羟乙基)哌嗪-N’-(2-乙磺酸)钠盐
C8H17N2NaO4S
260.29 先生
CAS [75277-39-3]
欧盟编号 278-169-7
MDL 编号 MFCD00036463
PubChem 物质 ID 24895780
pKa 7.5 在 25°C
HEPES钠的有用pH范围:6.6 – 8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C

HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

使用 HCl 将 HR2-733 滴定至 pH 7.5
HR2-733 测量的电导率范围:25°C 时 41.7 – 43.4 mS/cm
HR2-733 测量的折射率范围:20°C 时为 1.37577 – 1.37766

HR2-577 是 HEPES 钠的游离碱,使用 HCl 滴定至所需的 pH 值
HR2-577 测量的 pH 范围:25°C 时为 10.2 – 12.1
HR2-577 测量的电导率范围:25.9 – 27.2 mS/cm 在 25°C
HR2-577 测量的折射率范围:20°C 时为 1.37097 – 1.37298

HR2-931-01 – 1.0 M HEPES 钠 pH 6.8 是 Custom Shop 试剂,用于复制和优化与 Silver Bullets 试剂盒配合使用的结晶试剂。

HEPES sodium Buffer
CAT NO

HR2-577

NAME

1.0 M HEPES sodium

DESCRIPTION

100 mL

PRICE

$133.00

CAT NO

HR2-733

NAME

1.0 M HEPES sodium pH 7.5

DESCRIPTION

100 mL

PRICE

$133.00

相关项目

  • Hydrochloric acid
  • StockOptions Sodium HEPES Buffer Kit
  • Individual StockOptions Sodium HEPES Reagents
  • 盐酸
    StockOptions HEPES 钠缓冲液套件
    个别股票期权 HEPES 钠试剂

参考

1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.

1. GM2 激活蛋白的结晶和初步 X 射线表征。 C. S. 赖特和 S.-C。 李。 晶体学报。 (1997)。 D53,211-212。

Hampton蛋白结晶试剂盒Imidazole Buffer/HR2-573/HR2-819

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Imidazole Buffer

Imidazole Buffer

应用

  • Crystallization grade Imidazole for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级咪唑

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Imidazole

Synonyms: 1,3-Diaza-2,4-cyclopentadiene or Glyoxaline
C3H4N2
Mr 68.08
CAS Number [288-32-4]
EC Number 206-019-2
Beilstein Registry Number 103853
Merck 14,4912
RTECS NI3325000
MDL Number MFCD00005183
PubChem Substance ID 24880372
Purity ≥ 99.5%
UN Number 2923 8/PG 3

pKa = 6.95
The useful pH range of an Imidazole can be 6.2 – 7.8

HR2-819 is titrated to pH 7.0 using HCl
HR2-819 Measured Conductivity Range: 45.4 – 50.4 mS/cm at 25°C
HR2-819 Measured Refractive Index Range: 1.34836 – 1.34904 at 20°C

Titrate HR2-573 to the desired pH using HCl
HR2-573 Measured pH Range: 10.5 – 10.7 at 25°C
HR2-573 Measured Conductivity Range: 71.1 – 101.5 µS/cm at 25°C
HR2-573 Measured Refractive Index Range: 1.34420 – 1.34440 at 20°C

同义词:1,3-Diaza-2,4-cyclopentadiene 或 Glyoxaline
C3H4N2
68.08 先生
CAS 编号 [288-32-4]
欧盟编号 206-019-2
贝尔斯坦注册号 103853
默克 14,4912
RTECS NI3325000
MDL 编号 MFCD00005183
PubChem 物质 ID 24880372
纯度≥99.5%
联合国编号 2923 8/PG 3

pKa = 6.95
咪唑的有用 pH 范围可以是 6.2 – 7.8

HR2-819 使用 HCl 滴定至 pH 7.0
HR2-819 测得的电导率范围:25°C 时为 45.4 – 50.4 mS/cm
HR2-819 测量的折射率范围:20°C 时为 1.34836 – 1.34904

使用 HCl 将 HR2-573 滴定至所需的 pH 值
HR2-573 测得的 pH 范围:25°C 时 10.5 – 10.7
HR2-573 测得的电导率范围:25°C 时为 71.1 – 101.5 µS/cm
HR2-573 测量的折射率范围:20°C 时为 1.34420 – 1.34440

Ca  ≤0.001%
Cd  ≤0.0005%
Cl   ≤0.005%
Co  ≤0.0005%
Cr  ≤0.0005%
Cu  ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Zn  ≤0.0005%

Imidazole Buffer
CAT NO

HR2-573

NAME

1.0 M Imidazole

DESCRIPTION

100 mL

PRICE

$93.00

CAT NO

HR2-819

NAME

1.0 M Imidazole pH 7.0

DESCRIPTION

100 mL

PRICE

$111.00

相关项目

  • Hydrochloric acid
  • Individual StockOptions Imidazole Reagents
  • StockOptions Imidazole Kit
  • 盐酸
    个别股票期权 咪唑试剂
    StockOptions 咪唑试剂盒

参考

1. Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. X. Qiu and C. A. Janson. Acta Cryst. (2004). D60, 1545-1554 [doi:10.1107/S0907444904015422].

2. Protein crystallization by surface entropy reduction: optimization of the SER strategy. D. R. Cooper, T. Boczek, K. Grelewska, M. Pinkowska, M. Sikorska, M. Zawadzki and Z. Derewenda. Acta Cryst. (2007). D63, 636-645 [doi:10.1107/S0907444907010931].

1. apo 酰基载体蛋白的结构和通过金属离子设计蛋白质结晶的建议。 X. 邱和 C. A. Janson。 晶体学报。 (2004 年)。 D60, 1545-1554 [doi:10.1107/S0907444904015422]。

2. 表面熵减少的蛋白质结晶:SER 策略的优化。 D. R. Cooper、T. Boczek、K. Grelewska、M. Pinkowska、M. Sikorska、M. Zawadzki 和 Z. Derewenda。 晶体学报。 (2007 年)。 D63, 636-645 [doi:10.1107/S0907444907010931]。