Hampton蛋白结晶试剂盒Dialysis Buttons/HR3-336/HR3-316/HR3-332/HR3-330

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Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Buttons

Dialysis Buttons

应用

  • Crystallization by dialysis, protein folding, & small volume sample dialysis
  • 通过透析、蛋白质折叠和小体积样品透析进行结晶

特征

  • Low volume dialysis
  • Fits in 24 well plates
  • Use over and over again
  • 低容量透析
    适合 24 孔板
    反复使用

描述

In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3

Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.

在透析方法中,有问题的样品通过半透膜与“沉淀剂”分离,半透膜允许小试剂分子通过,但阻止生物大分子穿过膜。1-3

透析按钮由聚苯乙烯注塑成型,大小与小按钮相当。将样品放置在该腔室中,以便在按钮顶部形成一个轻微的液体圆顶。透析膜放置在按钮/样品的顶部,并用 O 形环固定到位。 O 形环由透析按钮中的凹槽固定到位。透析按钮配有 O 形圈。 5 µl 至 100 µl 大小的透析按钮的总高度约为 6 mm,外径约为 10 mm,未安装 O 形圈,直径约为 12 mm,安装了 O 形圈。 200 µl 和 350 µl 大小的透析按钮总高度约为 8 mm,外径约为 15 mm,不带 O 形圈,直径约为 17 mm,带 O 形圈。每个透析按钮包都包括一个塑料高尔夫球 T 形涂抹器,以帮助将透析膜应用到按钮上并使用随附的 O 形圈固定。

HR3-336 Dialysis Buttons Sampler

Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
Dialysis Buttons
CAT NO

HR3-336

NAME

Dialysis Buttons Sampler

DESCRIPTION

5 of each size listed below

PRICE

$105.00

CAT NO

HR3-314

NAME

5 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-316

NAME

10 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-318

NAME

15 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-320

NAME

20 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-326

NAME

50 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-328

NAME

100 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-330

NAME

200 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

CAT NO

HR3-332

NAME

350 µl Dialysis Button

DESCRIPTION

50 pack

PRICE

$105.00

支持材料

Dialysis Buttons Dialysis Button User Guide

相关项目

  • Dialysis Membrane Discs for Buttons
  • 用于按钮的透析膜盘

参考

1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).

2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).

3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.

4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.

1. McPherson, A.,蛋白质晶体的制备和分析,Krieger 出版社,88-91 (1992)。

2. Durcruix, A 和 Giege, R.,核酸和蛋白质的结晶,实用方法,牛津大学出版社,78-82 (1992)。

3. Zeppenzauer, M. 等人,Acta Chem Scan (1967) 21, 1009。

4. 呼吸复合体膜结构域的结构 I. Efremov RG, Sazanov LA。 自然。 2011 年 8 月 7 日;476(7361):414-20。 doi:10.1038/nature10330。